Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium

Yong Kwon Lee, Seung Yeul Kim, Ki Heon Kim, Bok Hwan Chun, Kweon Haeng Lee, Duk Jae Oh, Namhyun Chung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE.

Original languageEnglish
Pages (from-to)1931-1936
Number of pages6
JournalBiotechnology letters
Volume30
Issue number11
DOIs
Publication statusPublished - 2008 Nov

Keywords

  • Cell growth
  • Keratinocytes
  • Serum-free medium
  • Soybean protein hydrolysates

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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