Usefulness of frozen-thawed-deglycerolized red blood cells as quality control materials for red blood cell deformability test

Yu Kyung Kim, Dong Il Won, Hyun Ok Kim, Sehyun Shin, Jang Soo Suh

Research output: Contribution to journalArticle

Abstract

Background : The red blood cell (RBC) deformability test is a useful method for measuring the ability of RBCs to adapt their shape to the flow conditions. Using this test, several investigators have shown the relationship between RBC deformability and numerous clinical conditions. For the quality control (QC) of RBC deformability test, we evaluated whether frozen-thawed-deglycerolized RBCs can be used as QC materials. Methods : Packed RBCs were frozen with 40% (wt/vol) glycerol and stored at -80°C for 3 months. For 10 different frozen RBC panels, RBCs were thawed, deglycerolized and stored at 4°C for 4 weeks. Using microfluidic ektacytometer, we measured RBC deformability of the thawed RBCs. The stability of thawed RBCs was tested once a day for 28 days of storage time and was analyzed by simple regression analysis. The precision of the test using thawed RBCs was analyzed for 7 days of storage time by calculation of CV values of intra-assay (10 measurements/assay) and betweenday measurements. Results : Frozen-thawed-deglycerolized RBCs were stable for 1 week. Within-run and betweenday precisions of the RBC deformability test during 7 days of storage of thawed RBCs were 1.4-2.9%, and 1.9-2.8%, respectively. Conclusions : Frozen-thawed-deglycerolized RBCs used in RBC deformability test showed satisfactory within-run and between-run precisions and stability for one week after thawing, and may be used as QC materials for this test.

Original languageEnglish
Pages (from-to)697-701
Number of pages5
JournalKorean Journal of Laboratory Medicine
Volume30
Issue number6
DOIs
Publication statusPublished - 2010 Dec 1

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Keywords

  • Frozen RBC
  • QC
  • RBC deformability

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry

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