Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells

Jin Woo Lee, Dae Seok Na, Su Kyoung Chae, Choong Kim, Ji Yoon Kang, Byeong Kwon Ju, Hwang Lee, Sun Uk Kim, Chang Nam Hwang, Sang Ho Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The development of proper biomaterials is critical for the success of cell therapy and modern tissue engineering. Here, we extruded the yolk and remaining inner mass from fertilized zebrafish eggs and used the resulting chorions as a biomaterial for the differentiation and attachment of mouse P19 embryonic carcinoma (EC) cells. Cells inserted into the chorion showed the spontaneous formation of embryoid body due to the repulsive cell adhesion of the chorion and differentiated specifically into neural cells and cardiomyocytes. In contrast, dissolved chorion extracellular matrix (ECM) conferred enhanced cell attachment on it, suggesting that a unique property of the zebrafish chorion with nanoporous structure appears to be responsible for the simple and controllable embryoid formation for stem cell differentiation. These results indicate that chorions from fertilized zebrafish eggs may be used as an extracellular matrix alternative and applied for stem cell differentiation to specific cell lineages.

Original languageEnglish
Pages (from-to)7615-7620
Number of pages6
JournalLangmuir
Volume21
Issue number17
DOIs
Publication statusPublished - 2005 Aug 16

Fingerprint

Chorion
stem cells
Zygote
eggs
Biocompatible Materials
Zebrafish
Stem cells
Biomaterials
attachment
mice
Stem Cells
Cell adhesion
cells
Tissue engineering
Cells
Extracellular Matrix
Cell Differentiation
Embryoid Bodies
tissue engineering
Cell Lineage

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells. / Lee, Jin Woo; Na, Dae Seok; Chae, Su Kyoung; Kim, Choong; Kang, Ji Yoon; Ju, Byeong Kwon; Lee, Hwang; Kim, Sun Uk; Hwang, Chang Nam; Lee, Sang Ho.

In: Langmuir, Vol. 21, No. 17, 16.08.2005, p. 7615-7620.

Research output: Contribution to journalArticle

Lee, Jin Woo ; Na, Dae Seok ; Chae, Su Kyoung ; Kim, Choong ; Kang, Ji Yoon ; Ju, Byeong Kwon ; Lee, Hwang ; Kim, Sun Uk ; Hwang, Chang Nam ; Lee, Sang Ho. / Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells. In: Langmuir. 2005 ; Vol. 21, No. 17. pp. 7615-7620.
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