Utilization of agricultural residues for enhancement of cellulolytic enzyme production and enzymatic saccharification by Trichoderma harzianum KUC1716

Hanbyul Lee, Young Min Lee, Young Mok Heo, Jaejung Lee, Jun Seok Kim, Kyu Young Kang, Jae-Jin Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)


Agricultural residues are a potentially economical and eco-friendly source for second-generation ethanol production. In the present study, efficient cellulolytic enzyme production was studied using agricultural residues as a carbon source. Four types of agricultural residues were evaluated as carbon sources for Trichoderma harzianum KUC1716. Barley straw was identified as the optimal carbon source for the fungus such that the highest enzyme activity occurred in these cultures compared to cultures that used empty fruit bunches (EFB), miscanthus, or rice straw sources. T. harzianum KUC1716 produced high levels of filter paperase (FPase) and cellobiohydrolase (CBH) under optimized medium conditions when a central composite design and response surface methodology were used. Furthermore, T. harzianum KUC1716 fungal enzymes exhibited a significant hydrolysis yield for various types of agricultural residues compared to a commercial enzyme. Our study demonstrates that utilization of agricultural residues for both enzyme production and enzymatic saccharification will contribute to an increase in economical and eco-friendly production of biofuels and bio-based products.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalIndustrial Crops and Products
Publication statusPublished - 2017 Dec 15



  • Agricultural residue
  • Cellulase
  • Hydrolysis
  • Optimization
  • Response surface methodology (RSM)
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Agronomy and Crop Science

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