Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Hyun Yong Shin, Jin Young Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(l-α-aminoadipyl)-l-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalJournal of Biotechnology
Volume151
Issue number4
DOIs
Publication statusPublished - 2011 Feb 20

Keywords

  • Acremonium chrysogenum
  • Cephalosporin C
  • Cysteine
  • Methionine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture'. Together they form a unique fingerprint.

  • Cite this