Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin

Jin Choi Nag, Gyu Park Hui, Jun Kim Young, In-Hwan Kim, Soon Kang Hye, Suk Yoon Chil, Geun Yoon Ho, Su Il Park, Woo Lee Jae, Hyun Chung Soo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.

Original languageEnglish
Pages (from-to)10908-10912
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number22
DOIs
Publication statusPublished - 2008 Nov 26

Fingerprint

Bifidobacterium breve
Conjugated Linoleic Acids
conjugated linoleic acid
Substrates
Bifidobacterium
Starters
Dairy products
Monoglycerides
monolinolein
Dairy Products
monoacylglycerols
Linoleic Acid
starter cultures
Fermentation
dairy products
Intestines
linoleic acid
milk production
Milk
intestines

Keywords

  • Bifidobacterium breve
  • Conjugated linoleic acid
  • Milk fermentation
  • Monolinolein
  • Starter

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin. / Nag, Jin Choi; Hui, Gyu Park; Young, Jun Kim; Kim, In-Hwan; Hye, Soon Kang; Chil, Suk Yoon; Ho, Geun Yoon; Park, Su Il; Jae, Woo Lee; Soo, Hyun Chung.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 22, 26.11.2008, p. 10908-10912.

Research output: Contribution to journalArticle

Nag, Jin Choi ; Hui, Gyu Park ; Young, Jun Kim ; Kim, In-Hwan ; Hye, Soon Kang ; Chil, Suk Yoon ; Ho, Geun Yoon ; Park, Su Il ; Jae, Woo Lee ; Soo, Hyun Chung. / Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin. In: Journal of Agricultural and Food Chemistry. 2008 ; Vol. 56, No. 22. pp. 10908-10912.
@article{d5c42630ad634201a2b2b352b5cf4563,
title = "Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin",
abstract = "This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.",
keywords = "Bifidobacterium breve, Conjugated linoleic acid, Milk fermentation, Monolinolein, Starter",
author = "Nag, {Jin Choi} and Hui, {Gyu Park} and Young, {Jun Kim} and In-Hwan Kim and Hye, {Soon Kang} and Chil, {Suk Yoon} and Ho, {Geun Yoon} and Park, {Su Il} and Jae, {Woo Lee} and Soo, {Hyun Chung}",
year = "2008",
month = "11",
day = "26",
doi = "10.1021/jf801597t",
language = "English",
volume = "56",
pages = "10908--10912",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "22",

}

TY - JOUR

T1 - Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin

AU - Nag, Jin Choi

AU - Hui, Gyu Park

AU - Young, Jun Kim

AU - Kim, In-Hwan

AU - Hye, Soon Kang

AU - Chil, Suk Yoon

AU - Ho, Geun Yoon

AU - Park, Su Il

AU - Jae, Woo Lee

AU - Soo, Hyun Chung

PY - 2008/11/26

Y1 - 2008/11/26

N2 - This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.

AB - This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.

KW - Bifidobacterium breve

KW - Conjugated linoleic acid

KW - Milk fermentation

KW - Monolinolein

KW - Starter

UR - http://www.scopus.com/inward/record.url?scp=57849150933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57849150933&partnerID=8YFLogxK

U2 - 10.1021/jf801597t

DO - 10.1021/jf801597t

M3 - Article

C2 - 18973338

AN - SCOPUS:57849150933

VL - 56

SP - 10908

EP - 10912

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 22

ER -