Validation and application of HPLC-ESI-MS/MS method for the determination of irsogladine

N. H. Hoang, N. L. Huong, S. Y. Hong, Je Won Park

Research output: Contribution to journalArticlepeer-review

Abstract

A highly sensitive analytical tool for the fast quantification of irsogladine in human plasma was developed. Cleanup using a solid-phase extraction technique is a simple method for extracting both irsogladine and lamotrigine (internal standard) spiked into human plasma. The resolvable separation of both analytes through reversed-phase high-performance liquid chromatography (HPLC) was carried out within 5 min. The HPLC-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) method, which was operated in a selected reaction monitoring mode specific to the target analytes, was verified for use in the quantification of irsogladine. The inter- and intra-day precision (relative standard deviation, RSD) of irsogladine spiked into quality control samples were <7%, and their accuracies were between 96.6% and 102.1%. The calibration curve for irsogladine spiked into human plasma was linear over the range from 1.8 to 100 ng mL−1 with lower limit of quantification at 1.8 ng mL−1. The established method was successfully applied for a bioequivalence study of irsogladine.

Original languageEnglish
Pages (from-to)459-462
Number of pages4
JournalActa Chromatographica
Volume29
Issue number4
DOIs
Publication statusPublished - 2017 Dec

Keywords

  • Bioequivalence study
  • HPLC-ESI-MS/MS
  • Human plasma
  • Irsogladine

ASJC Scopus subject areas

  • Chemistry(all)

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