Validation of the metabolic pathway of the alginate-derived monomer in Saccharophagus degradans 2-40T by gas chromatography–mass spectrometry

Do Hyoung Kim, Damao Wang, Eun Ju Yun, Sooah Kim, Soo Rin Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Marine macroalgae are potential resources for the sustainable production of biofuels and bio-based chemicals. Alginate, a major component of brown macroalgae, consists of two uronate monomers, which are further non-enzymatically converted to 4-deoxy-L-erythro-5-hexoseulose uronate (DEH). In several marine bacteria, DEH is known to be metabolized via three enzymatic steps, consisting of DEH reductase, 2-keto-3-deoxy-D-gluconate (KDG) kinase, and 2-keto-3-deoxy-phosphogluconate (KDPG) aldolase, which yields two glycolytic intermediates: D-glyceraldehyde-3-phosphate and pyruvate. However, such functions of these enzymes for the DEH pathway have rarely been experimentally validated. In the present study, the DEH metabolic pathway was investigated in Saccharophagus degradans 2-40T, a marine bacterium that utilizes alginate. Through in vitro tests assisted by gas chromatography/mass spectrometry and gas chromatography/time-of-flight mass spectrometry, the purified enzymes were functionally confirmed and annotated as dehR, kdgK, and kdpgA, respectively. In conclusion, we report the in vitro validation of the metabolic pathway of DEH monomerized from alginate.

Original languageEnglish
Pages (from-to)1374-1379
Number of pages6
JournalProcess Biochemistry
Volume51
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Alginate
Metabolic Networks and Pathways
Spectrometry
Seaweed
Spectrum Analysis
Monomers
Gases
gluconokinase
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Bacteria
Enzymes
Glyceraldehyde 3-Phosphate
Fructose-Bisphosphate Aldolase
Biofuels
Pyruvic Acid
Mass Spectrometry
Oxidoreductases
Phosphates

Keywords

  • 2-Keto-3-deoxy-D-gluconate
  • 2-Keto-3-deoxy-phosphogluconate
  • 4-Deoxy-L-erythro-5-hexoseulose uronate
  • Alginate metabolism
  • Saccharophagus degradans 2-40
  • Short-chain dehydrogenase/reductase

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Validation of the metabolic pathway of the alginate-derived monomer in Saccharophagus degradans 2-40T by gas chromatography–mass spectrometry. / Kim, Do Hyoung; Wang, Damao; Yun, Eun Ju; Kim, Sooah; Kim, Soo Rin; Kim, Kyoung Heon.

In: Process Biochemistry, Vol. 51, No. 10, 01.10.2016, p. 1374-1379.

Research output: Contribution to journalArticle

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