Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

Bo Wang, Myat Htut Nyunt, Seung Gyu Yun, Feng Lu, Yang Cheng, Jin Hee Han, Kwon Soo Ha, Won Sun Park, Seok Ho Hong, Chae Seung Lim, Jun Cao, Jetsumon Sattabongkot, Myat Phone Kyaw, Liwang Cui, Eun Taek Han

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Abstract

The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalActa Tropica
Volume170
DOIs
Publication statusPublished - 2017 Jun 1

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Keywords

  • Heterozygosity
  • Linkage disequilibrium
  • Malaria
  • Variable number of tandem repeats (VNTR)

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Wang, B., Nyunt, M. H., Yun, S. G., Lu, F., Cheng, Y., Han, J. H., Ha, K. S., Park, W. S., Hong, S. H., Lim, C. S., Cao, J., Sattabongkot, J., Kyaw, M. P., Cui, L., & Han, E. T. (2017). Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates. Acta Tropica, 170, 161-168. https://doi.org/10.1016/j.actatropica.2017.01.013