Variation in pathogenicity of a mountain pine beetle-associated blue-stain fungus, Grosmannia clavigera, on young lodgepole pine in British Columbia

Alex Plattner, Jae-Jin Kim, Scott DiGuistini, Colette Breuil

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.

Original languageEnglish
Pages (from-to)457-466
Number of pages10
JournalCanadian Journal of Plant Pathology
Volume30
Issue number3
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Grosmannia
blue-stain fungi
Brickellia
Dendroctonus ponderosae
Pinus contorta var. latifolia
British Columbia
sapwood
pathogenicity
lesions (plant)
water content
Pinus contorta
phloem
signs and symptoms (plants)
oxygen
fungi

Keywords

  • Blue-stain fungus
  • Grosmannia clavigera
  • Intraspecific variation
  • Mountain pine beetle
  • Ophiostoma clavigerum
  • Pathogenicity

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Variation in pathogenicity of a mountain pine beetle-associated blue-stain fungus, Grosmannia clavigera, on young lodgepole pine in British Columbia. / Plattner, Alex; Kim, Jae-Jin; DiGuistini, Scott; Breuil, Colette.

In: Canadian Journal of Plant Pathology, Vol. 30, No. 3, 01.09.2008, p. 457-466.

Research output: Contribution to journalArticle

@article{04d67c0848284793962e908d2c5d0663,
title = "Variation in pathogenicity of a mountain pine beetle-associated blue-stain fungus, Grosmannia clavigera, on young lodgepole pine in British Columbia",
abstract = "Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.",
keywords = "Blue-stain fungus, Grosmannia clavigera, Intraspecific variation, Mountain pine beetle, Ophiostoma clavigerum, Pathogenicity",
author = "Alex Plattner and Jae-Jin Kim and Scott DiGuistini and Colette Breuil",
year = "2008",
month = "9",
day = "1",
doi = "10.1080/07060660809507543",
language = "English",
volume = "30",
pages = "457--466",
journal = "Canadian Journal of Plant Pathology",
issn = "0706-0661",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - Variation in pathogenicity of a mountain pine beetle-associated blue-stain fungus, Grosmannia clavigera, on young lodgepole pine in British Columbia

AU - Plattner, Alex

AU - Kim, Jae-Jin

AU - DiGuistini, Scott

AU - Breuil, Colette

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.

AB - Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.

KW - Blue-stain fungus

KW - Grosmannia clavigera

KW - Intraspecific variation

KW - Mountain pine beetle

KW - Ophiostoma clavigerum

KW - Pathogenicity

UR - http://www.scopus.com/inward/record.url?scp=77953232153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953232153&partnerID=8YFLogxK

U2 - 10.1080/07060660809507543

DO - 10.1080/07060660809507543

M3 - Article

VL - 30

SP - 457

EP - 466

JO - Canadian Journal of Plant Pathology

JF - Canadian Journal of Plant Pathology

SN - 0706-0661

IS - 3

ER -