Vascular endothelial growth factor induces endothelin-1 production via matrix metalloproteinase-2 rather than endothelin-converting enzyme-1

Kyung Ju Lee, Meyoung-Kon Kim, Yoon Hee Park, Hyun Joo Seol, Ji Eun Lim, Jeong No Lee, Min Jeong Oh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To investigate the mechanism of vascular endothelial growth factor (VEGF)-induced endothelin-1 production in human umbilical vein endothelial cells (HUVECs). Methods: Endothelin-1 levels were measured in conditioned medium of women with preeclampsia HUVECs were treated with different concentrations of VEGF165 and at various time intervals. Next, we measured endothelin-1 levels after HUVECs were also incubated with VEGF and endothelin-converting enzyme-1 (ECE-1) inhibitor or tissue inhibitors of matrix metalloproteinase-2 (TIMP-2). Additionally, the circulating levels of total and free VEGF, matrix metalloproteinase-2 (MMP-2), and endothelin-1 were measured in 20 preeclamptic patients and 20 healthy pregnant controls. Results: HUVECs treated with VEGF increased their endothelin-1 production in a concentration and time-dependent manner. The production of endothelin-1 was inhibited by TIMP-2, but not by the ECE-1 inhibitor. Total VEGF, MMP-2, and endothelin-1 concentrations were higher in preeclampsia and showed significant positive correlations between them. Conclusion: These findings suggest that VEGF-induced endothelin-1 production might be mediated by MMP-2 rather than by ECE-1 upregulation.

Original languageEnglish
Pages (from-to)189-199
Number of pages11
JournalHypertension in Pregnancy
Volume26
Issue number2
DOIs
Publication statusPublished - 2007 Apr 1

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Matrix Metalloproteinase 2
Endothelin-1
Vascular Endothelial Growth Factor A
Human Umbilical Vein Endothelial Cells
Tissue Inhibitor of Metalloproteinase-2
Enzyme Inhibitors
Pre-Eclampsia
Endothelin-Converting Enzymes
Conditioned Culture Medium
Up-Regulation

Keywords

  • Endothelin-1
  • Endothelin-converting enzyme-1
  • Matrix metalloproteinase-2
  • Preeclampsia
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Internal Medicine

Cite this

Vascular endothelial growth factor induces endothelin-1 production via matrix metalloproteinase-2 rather than endothelin-converting enzyme-1. / Lee, Kyung Ju; Kim, Meyoung-Kon; Park, Yoon Hee; Seol, Hyun Joo; Lim, Ji Eun; Lee, Jeong No; Oh, Min Jeong.

In: Hypertension in Pregnancy, Vol. 26, No. 2, 01.04.2007, p. 189-199.

Research output: Contribution to journalArticle

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