Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices

Seung Woo Cho, Hee Jung Park, Ju Hee Ryu, Soo Hyun Kim, Young Ha Kim, Cha Yong Choi, Min Jae Lee, Jong Sung Kim, In Sung Jang, Dong Ik Kim, Byung Soo Kim

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Synthetic polymer vascular patches used in cardiovascular surgery have shortcomings such as thrombosis, intimal hyperplasia, calcification, infection, and no growth potential. Tissue-engineered vascular patches using autologous vascular cells may solve these problems. In this study, we developed a tissue-engineered vascular patch using autologous bone marrow-derived cells (BMCs) and decellularized tissue matrices. Vascular smooth muscle cells and endothelial cells were differentiated from bone marrow mononuclear cells in vitro. Tissue-engineered vascular patches were fabricated by seeding these cells onto decellularized canine inferior vena cava matrices and implanted into the inferior vena cava of dogs. Three weeks after implantation, the tissue-engineered vascular patches were patent with no sign of thrombus formation. Histological, immunohistochemical, and electron microscopic analyses of the vascular patches retrieved 3 weeks after implantation revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular patches, indicating that the BMCs survived after implantation and contributed to the vascular tissue regeneration. This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and decellularized tissue matrices.

Original languageEnglish
Pages (from-to)1915-1924
Number of pages10
JournalBiomaterials
Volume26
Issue number14
DOIs
Publication statusPublished - 2005 May 1
Externally publishedYes

Fingerprint

Bone Marrow Cells
Blood Vessels
Bone
Tissue
Muscle
Cardiovascular surgery
Inferior Vena Cava
Elastin
Tissue regeneration
Regeneration
Thrombosis
Endothelial cells
Fluorescent Dyes
Tunica Intima
Collagen
Polymers
Dyes
Cells
Vascular Smooth Muscle
Smooth Muscle Myocytes

Keywords

  • Bone marrow-derived cell
  • Decellularized tissue matrix
  • Tissue engineering
  • Vascular patch

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Cho, S. W., Park, H. J., Ryu, J. H., Kim, S. H., Kim, Y. H., Choi, C. Y., ... Kim, B. S. (2005). Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices. Biomaterials, 26(14), 1915-1924. https://doi.org/10.1016/j.biomaterials.2004.06.018

Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices. / Cho, Seung Woo; Park, Hee Jung; Ryu, Ju Hee; Kim, Soo Hyun; Kim, Young Ha; Choi, Cha Yong; Lee, Min Jae; Kim, Jong Sung; Jang, In Sung; Kim, Dong Ik; Kim, Byung Soo.

In: Biomaterials, Vol. 26, No. 14, 01.05.2005, p. 1915-1924.

Research output: Contribution to journalArticle

Cho, SW, Park, HJ, Ryu, JH, Kim, SH, Kim, YH, Choi, CY, Lee, MJ, Kim, JS, Jang, IS, Kim, DI & Kim, BS 2005, 'Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices', Biomaterials, vol. 26, no. 14, pp. 1915-1924. https://doi.org/10.1016/j.biomaterials.2004.06.018
Cho, Seung Woo ; Park, Hee Jung ; Ryu, Ju Hee ; Kim, Soo Hyun ; Kim, Young Ha ; Choi, Cha Yong ; Lee, Min Jae ; Kim, Jong Sung ; Jang, In Sung ; Kim, Dong Ik ; Kim, Byung Soo. / Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices. In: Biomaterials. 2005 ; Vol. 26, No. 14. pp. 1915-1924.
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