Vasoactive intestinal peptide stimulates turkey prolactin gene expression by increasing transcription rate and enhancing mRNA stability

Z. Tong, G. R. Pitts, S. You, D. N. Foster, M. E. El Halawani

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study evaluates the transcriptional and post-transcriptional regulation of prolactin (PRL) by vasoactive intestinal peptide (VIP). Pituitary nuclei from laying (control), incubating (with enhanced VIP secretion), and VIP-immunized laying turkey hens, and from pituitary cells cultured with or without VIP were used in nuclear run-on transcription assays. Cytoplasmic PRL mRNA was analyzed by slot blot hybridization. PRL transcription was greater in hyperprolactinemic incubating birds (PRL/β- actin=3.33) than in laying birds (PRL/β-actin=1.83). VIP-immunoneutralized birds had 47% and 51% decreases in PRL transcription and cytoplasmic PRL mRNA, respectively when compared with laying birds. In primary pituitary cell cultures, VIP significantly increased the transcription rate of PRL (3.8- fold) and cytoplasmic PRL mRNA (3.2-fold) compared with that of non-VIP- treated pituitary cells. The stability of pre-existing PRL mRNA was measured by Northern blot analysis after addition of actinomycin D. PRL mRNA half, lives were calculated using a two-component model, with a first-long component of 18.0 ± 1.0 h and a second-short component of 3.7 ± 0.7 h in non-VIP-treated pituitary cells. Both half-lives were significantly increased (53.2 ± 6.9 and 26.3 ± 4.3 h) in VIP-treated cells. The present data show that VIP acts to stimulate PRL expression by up-regulating the transcription rate of PRL and by enhancing PRL mRNA stability.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalJournal of Molecular Endocrinology
Volume21
Issue number3
DOIs
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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