Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection

Yoon Ho Roh, Sang Yun Yeom, Jungkyu Choi, Il Joo Cho, Nakwon Choi, Ki Wan Bong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Encoded hydrogel particles have drawn many attentions in diagnostics as the particles can be used for high-performance multiplexed assays. Here, we present encoded tetragonal hydrogel microparticles that are comprised of vertically distinct code and probe regions, and incorporated with quantum dots (QDs) in the code regions. By virtue of the particle geometry, particles can be synthesized at a high production rate in vertically stacked micro-flows using hydrodynamic focusing lithography. The particle coding capacity can be largely expanded by changing wavelengths of QDs and the number of code layers. We also demonstrate the multiplexed capability of the particles in a DNA assay.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1407-1409
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Fingerprint

Biomolecules
Hydrogels
Semiconductor quantum dots
Assays
Lithography
Particles (particulate matter)
DNA
Hydrodynamics
Wavelength
Geometry

Keywords

  • Hydrodynamic focusing lithography (HFL)
  • Multiplexed assay
  • Quantum dots (QDs)

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Roh, Y. H., Yeom, S. Y., Choi, J., Cho, I. J., Choi, N., & Bong, K. W. (2015). Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1407-1409). Chemical and Biological Microsystems Society.

Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection. / Roh, Yoon Ho; Yeom, Sang Yun; Choi, Jungkyu; Cho, Il Joo; Choi, Nakwon; Bong, Ki Wan.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 1407-1409.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Roh, YH, Yeom, SY, Choi, J, Cho, IJ, Choi, N & Bong, KW 2015, Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1407-1409, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.
Roh YH, Yeom SY, Choi J, Cho IJ, Choi N, Bong KW. Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 1407-1409
Roh, Yoon Ho ; Yeom, Sang Yun ; Choi, Jungkyu ; Cho, Il Joo ; Choi, Nakwon ; Bong, Ki Wan. / Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 1407-1409
@inproceedings{e3d0ad803b6e4767a56380ef1bb9874f,
title = "Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection",
abstract = "Encoded hydrogel particles have drawn many attentions in diagnostics as the particles can be used for high-performance multiplexed assays. Here, we present encoded tetragonal hydrogel microparticles that are comprised of vertically distinct code and probe regions, and incorporated with quantum dots (QDs) in the code regions. By virtue of the particle geometry, particles can be synthesized at a high production rate in vertically stacked micro-flows using hydrodynamic focusing lithography. The particle coding capacity can be largely expanded by changing wavelengths of QDs and the number of code layers. We also demonstrate the multiplexed capability of the particles in a DNA assay.",
keywords = "Hydrodynamic focusing lithography (HFL), Multiplexed assay, Quantum dots (QDs)",
author = "Roh, {Yoon Ho} and Yeom, {Sang Yun} and Jungkyu Choi and Cho, {Il Joo} and Nakwon Choi and Bong, {Ki Wan}",
year = "2015",
language = "English",
pages = "1407--1409",
booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Vertically encoded tetragonal hydrogel microparticles for multiplexed biomolecule detection

AU - Roh, Yoon Ho

AU - Yeom, Sang Yun

AU - Choi, Jungkyu

AU - Cho, Il Joo

AU - Choi, Nakwon

AU - Bong, Ki Wan

PY - 2015

Y1 - 2015

N2 - Encoded hydrogel particles have drawn many attentions in diagnostics as the particles can be used for high-performance multiplexed assays. Here, we present encoded tetragonal hydrogel microparticles that are comprised of vertically distinct code and probe regions, and incorporated with quantum dots (QDs) in the code regions. By virtue of the particle geometry, particles can be synthesized at a high production rate in vertically stacked micro-flows using hydrodynamic focusing lithography. The particle coding capacity can be largely expanded by changing wavelengths of QDs and the number of code layers. We also demonstrate the multiplexed capability of the particles in a DNA assay.

AB - Encoded hydrogel particles have drawn many attentions in diagnostics as the particles can be used for high-performance multiplexed assays. Here, we present encoded tetragonal hydrogel microparticles that are comprised of vertically distinct code and probe regions, and incorporated with quantum dots (QDs) in the code regions. By virtue of the particle geometry, particles can be synthesized at a high production rate in vertically stacked micro-flows using hydrodynamic focusing lithography. The particle coding capacity can be largely expanded by changing wavelengths of QDs and the number of code layers. We also demonstrate the multiplexed capability of the particles in a DNA assay.

KW - Hydrodynamic focusing lithography (HFL)

KW - Multiplexed assay

KW - Quantum dots (QDs)

UR - http://www.scopus.com/inward/record.url?scp=84983249275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983249275&partnerID=8YFLogxK

M3 - Conference contribution

SP - 1407

EP - 1409

BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

ER -