Vibrio vulnificus rtxE is important for virulence, and its expression is induced by exposure to host cells

Byung Cheon Lee, Hyun Lee Jeong, Won Kim Myung, Sik Kim Byoung, Hwan Oh Man, Kun Soo Kim, Tae Sung Kim, Ho Choi Sang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Numerous secreted virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. A mutant of V. vulnificus that exhibited less cytotoxicity to INT-407 human intestinal epithelial cells was screened from a library of mutants constructed by random transposon mutagen-esis. A transposon-tagging method was used to identify and clone an open reading frame encoding an RTX toxin secretion ATP binding protein, RtxE, from V. vulnificus. The deduced amino acid sequence of RtxE from V. vulnificus was 91% identical to that reported from Vibrio cholerae. Functions of the rtxE gene in virulence were assessed by constructing an isogenic mutant whose rtxE gene was inactivated by allelic exchanges and by evaluating the differences between its virulence phenotype and that of the wild type in vitro and in mice. The disruption of rtxE blocked secretion of RtxA to the cell exterior and resulted in a significant reduction in cytotoxic activity against epithelial cells in vitro. Also, the intraperitoneal 50% lethal dose of the rtxE mutant was 104 to 105 times higher than that of the parental wild type, indicating that RtxE is essential for the virulence of V. vulnificus. Furthermore, the present study demonstrated that the rtxBDE genes are transcribed as one transcriptional unit under the control of a single promoter, PrtxBDE. The activity of V. vulnificus PrtxBDE is induced by exposure to INT-407 cells, and the induction requires direct contact of the bacteria with the host cells.

Original languageEnglish
Pages (from-to)1509-1517
Number of pages9
JournalInfection and Immunity
Volume76
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1

Fingerprint

Vibrio vulnificus
Virulence
Epithelial Cells
Genes
Vibrio cholerae
Lethal Dose 50
Mutagens
Virulence Factors
Open Reading Frames
Libraries
Amino Acid Sequence
Carrier Proteins
Clone Cells
Adenosine Triphosphate
Bacteria
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Vibrio vulnificus rtxE is important for virulence, and its expression is induced by exposure to host cells. / Lee, Byung Cheon; Jeong, Hyun Lee; Myung, Won Kim; Byoung, Sik Kim; Man, Hwan Oh; Kim, Kun Soo; Kim, Tae Sung; Sang, Ho Choi.

In: Infection and Immunity, Vol. 76, No. 4, 01.04.2008, p. 1509-1517.

Research output: Contribution to journalArticle

Lee, Byung Cheon ; Jeong, Hyun Lee ; Myung, Won Kim ; Byoung, Sik Kim ; Man, Hwan Oh ; Kim, Kun Soo ; Kim, Tae Sung ; Sang, Ho Choi. / Vibrio vulnificus rtxE is important for virulence, and its expression is induced by exposure to host cells. In: Infection and Immunity. 2008 ; Vol. 76, No. 4. pp. 1509-1517.
@article{dcd74fc46d9f492684fdb8d23305c7db,
title = "Vibrio vulnificus rtxE is important for virulence, and its expression is induced by exposure to host cells",
abstract = "Numerous secreted virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. A mutant of V. vulnificus that exhibited less cytotoxicity to INT-407 human intestinal epithelial cells was screened from a library of mutants constructed by random transposon mutagen-esis. A transposon-tagging method was used to identify and clone an open reading frame encoding an RTX toxin secretion ATP binding protein, RtxE, from V. vulnificus. The deduced amino acid sequence of RtxE from V. vulnificus was 91{\%} identical to that reported from Vibrio cholerae. Functions of the rtxE gene in virulence were assessed by constructing an isogenic mutant whose rtxE gene was inactivated by allelic exchanges and by evaluating the differences between its virulence phenotype and that of the wild type in vitro and in mice. The disruption of rtxE blocked secretion of RtxA to the cell exterior and resulted in a significant reduction in cytotoxic activity against epithelial cells in vitro. Also, the intraperitoneal 50{\%} lethal dose of the rtxE mutant was 104 to 105 times higher than that of the parental wild type, indicating that RtxE is essential for the virulence of V. vulnificus. Furthermore, the present study demonstrated that the rtxBDE genes are transcribed as one transcriptional unit under the control of a single promoter, PrtxBDE. The activity of V. vulnificus PrtxBDE is induced by exposure to INT-407 cells, and the induction requires direct contact of the bacteria with the host cells.",
author = "Lee, {Byung Cheon} and Jeong, {Hyun Lee} and Myung, {Won Kim} and Byoung, {Sik Kim} and Man, {Hwan Oh} and Kim, {Kun Soo} and Kim, {Tae Sung} and Sang, {Ho Choi}",
year = "2008",
month = "4",
day = "1",
doi = "10.1128/IAI.01503-07",
language = "English",
volume = "76",
pages = "1509--1517",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Vibrio vulnificus rtxE is important for virulence, and its expression is induced by exposure to host cells

AU - Lee, Byung Cheon

AU - Jeong, Hyun Lee

AU - Myung, Won Kim

AU - Byoung, Sik Kim

AU - Man, Hwan Oh

AU - Kim, Kun Soo

AU - Kim, Tae Sung

AU - Sang, Ho Choi

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Numerous secreted virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. A mutant of V. vulnificus that exhibited less cytotoxicity to INT-407 human intestinal epithelial cells was screened from a library of mutants constructed by random transposon mutagen-esis. A transposon-tagging method was used to identify and clone an open reading frame encoding an RTX toxin secretion ATP binding protein, RtxE, from V. vulnificus. The deduced amino acid sequence of RtxE from V. vulnificus was 91% identical to that reported from Vibrio cholerae. Functions of the rtxE gene in virulence were assessed by constructing an isogenic mutant whose rtxE gene was inactivated by allelic exchanges and by evaluating the differences between its virulence phenotype and that of the wild type in vitro and in mice. The disruption of rtxE blocked secretion of RtxA to the cell exterior and resulted in a significant reduction in cytotoxic activity against epithelial cells in vitro. Also, the intraperitoneal 50% lethal dose of the rtxE mutant was 104 to 105 times higher than that of the parental wild type, indicating that RtxE is essential for the virulence of V. vulnificus. Furthermore, the present study demonstrated that the rtxBDE genes are transcribed as one transcriptional unit under the control of a single promoter, PrtxBDE. The activity of V. vulnificus PrtxBDE is induced by exposure to INT-407 cells, and the induction requires direct contact of the bacteria with the host cells.

AB - Numerous secreted virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. A mutant of V. vulnificus that exhibited less cytotoxicity to INT-407 human intestinal epithelial cells was screened from a library of mutants constructed by random transposon mutagen-esis. A transposon-tagging method was used to identify and clone an open reading frame encoding an RTX toxin secretion ATP binding protein, RtxE, from V. vulnificus. The deduced amino acid sequence of RtxE from V. vulnificus was 91% identical to that reported from Vibrio cholerae. Functions of the rtxE gene in virulence were assessed by constructing an isogenic mutant whose rtxE gene was inactivated by allelic exchanges and by evaluating the differences between its virulence phenotype and that of the wild type in vitro and in mice. The disruption of rtxE blocked secretion of RtxA to the cell exterior and resulted in a significant reduction in cytotoxic activity against epithelial cells in vitro. Also, the intraperitoneal 50% lethal dose of the rtxE mutant was 104 to 105 times higher than that of the parental wild type, indicating that RtxE is essential for the virulence of V. vulnificus. Furthermore, the present study demonstrated that the rtxBDE genes are transcribed as one transcriptional unit under the control of a single promoter, PrtxBDE. The activity of V. vulnificus PrtxBDE is induced by exposure to INT-407 cells, and the induction requires direct contact of the bacteria with the host cells.

UR - http://www.scopus.com/inward/record.url?scp=42149186570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149186570&partnerID=8YFLogxK

U2 - 10.1128/IAI.01503-07

DO - 10.1128/IAI.01503-07

M3 - Article

C2 - 18250174

AN - SCOPUS:42149186570

VL - 76

SP - 1509

EP - 1517

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 4

ER -