Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo

In Hwang Kim, Ik Jung Kim, Yancheng Wen, Na Young Park, Jinyoung Park, Keun Woo Lee, Ara Koh, Ji Hyun Lee, Seung-Hoi Koo, Kun Soo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We describe a novel insulin-degrading enzyme, SidC, that contributes to the proliferation of the human bacterial pathogen Vibrio vulnificus in a mouse model. SidC is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. Purified SidC causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. A comparison of mice infected with wild type V. vulnificus or an isogenic sidC-deletion strain showed that wild type bacteria proliferated to higher levels. Additionally, hyperglycemia leads to increased proliferation of V. vulnificus in diabetic mice. Consistent with these observations, the sid operon was up-regulated in response to low glucose levels through binding of the cAMP-receptor protein (CRP) complex to a region upstream of the operon. We conclude that glucose levels are important for the survival of V. vulnificus in the host, and that this pathogen uses SidC to actively manipulate host endocrine signals, making the host environment more favorable for bacterial survival and growth.

Original languageEnglish
Pages (from-to)18708-18720
Number of pages13
JournalJournal of Biological Chemistry
Volume290
Issue number30
DOIs
Publication statusPublished - 2015 Jul 24

Fingerprint

Insulysin
Vibrio vulnificus
Pathogens
Cyclic AMP Receptor Protein
Glucose
Operon
Blood Glucose
Bacteria
Insulin
Survival
Hyperglycemia
Growth
Infection
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kim, I. H., Kim, I. J., Wen, Y., Park, N. Y., Park, J., Lee, K. W., ... Kim, K. S. (2015). Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo. Journal of Biological Chemistry, 290(30), 18708-18720. https://doi.org/10.1074/jbc.M115.656306

Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo. / Kim, In Hwang; Kim, Ik Jung; Wen, Yancheng; Park, Na Young; Park, Jinyoung; Lee, Keun Woo; Koh, Ara; Lee, Ji Hyun; Koo, Seung-Hoi; Kim, Kun Soo.

In: Journal of Biological Chemistry, Vol. 290, No. 30, 24.07.2015, p. 18708-18720.

Research output: Contribution to journalArticle

Kim, IH, Kim, IJ, Wen, Y, Park, NY, Park, J, Lee, KW, Koh, A, Lee, JH, Koo, S-H & Kim, KS 2015, 'Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo', Journal of Biological Chemistry, vol. 290, no. 30, pp. 18708-18720. https://doi.org/10.1074/jbc.M115.656306
Kim, In Hwang ; Kim, Ik Jung ; Wen, Yancheng ; Park, Na Young ; Park, Jinyoung ; Lee, Keun Woo ; Koh, Ara ; Lee, Ji Hyun ; Koo, Seung-Hoi ; Kim, Kun Soo. / Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 30. pp. 18708-18720.
@article{91becf851dc54dab8b2799408e706d06,
title = "Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo",
abstract = "We describe a novel insulin-degrading enzyme, SidC, that contributes to the proliferation of the human bacterial pathogen Vibrio vulnificus in a mouse model. SidC is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. Purified SidC causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. A comparison of mice infected with wild type V. vulnificus or an isogenic sidC-deletion strain showed that wild type bacteria proliferated to higher levels. Additionally, hyperglycemia leads to increased proliferation of V. vulnificus in diabetic mice. Consistent with these observations, the sid operon was up-regulated in response to low glucose levels through binding of the cAMP-receptor protein (CRP) complex to a region upstream of the operon. We conclude that glucose levels are important for the survival of V. vulnificus in the host, and that this pathogen uses SidC to actively manipulate host endocrine signals, making the host environment more favorable for bacterial survival and growth.",
author = "Kim, {In Hwang} and Kim, {Ik Jung} and Yancheng Wen and Park, {Na Young} and Jinyoung Park and Lee, {Keun Woo} and Ara Koh and Lee, {Ji Hyun} and Seung-Hoi Koo and Kim, {Kun Soo}",
year = "2015",
month = "7",
day = "24",
doi = "10.1074/jbc.M115.656306",
language = "English",
volume = "290",
pages = "18708--18720",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "30",

}

TY - JOUR

T1 - Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo

AU - Kim, In Hwang

AU - Kim, Ik Jung

AU - Wen, Yancheng

AU - Park, Na Young

AU - Park, Jinyoung

AU - Lee, Keun Woo

AU - Koh, Ara

AU - Lee, Ji Hyun

AU - Koo, Seung-Hoi

AU - Kim, Kun Soo

PY - 2015/7/24

Y1 - 2015/7/24

N2 - We describe a novel insulin-degrading enzyme, SidC, that contributes to the proliferation of the human bacterial pathogen Vibrio vulnificus in a mouse model. SidC is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. Purified SidC causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. A comparison of mice infected with wild type V. vulnificus or an isogenic sidC-deletion strain showed that wild type bacteria proliferated to higher levels. Additionally, hyperglycemia leads to increased proliferation of V. vulnificus in diabetic mice. Consistent with these observations, the sid operon was up-regulated in response to low glucose levels through binding of the cAMP-receptor protein (CRP) complex to a region upstream of the operon. We conclude that glucose levels are important for the survival of V. vulnificus in the host, and that this pathogen uses SidC to actively manipulate host endocrine signals, making the host environment more favorable for bacterial survival and growth.

AB - We describe a novel insulin-degrading enzyme, SidC, that contributes to the proliferation of the human bacterial pathogen Vibrio vulnificus in a mouse model. SidC is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. Purified SidC causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. A comparison of mice infected with wild type V. vulnificus or an isogenic sidC-deletion strain showed that wild type bacteria proliferated to higher levels. Additionally, hyperglycemia leads to increased proliferation of V. vulnificus in diabetic mice. Consistent with these observations, the sid operon was up-regulated in response to low glucose levels through binding of the cAMP-receptor protein (CRP) complex to a region upstream of the operon. We conclude that glucose levels are important for the survival of V. vulnificus in the host, and that this pathogen uses SidC to actively manipulate host endocrine signals, making the host environment more favorable for bacterial survival and growth.

UR - http://www.scopus.com/inward/record.url?scp=84937696838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937696838&partnerID=8YFLogxK

U2 - 10.1074/jbc.M115.656306

DO - 10.1074/jbc.M115.656306

M3 - Article

C2 - 26041774

AN - SCOPUS:84937696838

VL - 290

SP - 18708

EP - 18720

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 30

ER -