Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses

Raquel Muñoz-Moreno, Carles Martínez-Romero, Daniel Blanco-Melo, Christian V. Forst, Raffael Nachbagauer, Asiel Arturo Benitez, Ignacio Mena, Sadaf Aslam, Vinod Balasubramaniam, Ilseob Lee, Maryline Panis, Juan Ayllón, David Sachs, Man Seong Park, Florian Krammer, Benjamin R. tenOever, Adolfo García-Sastre

Research output: Contribution to journalArticle

Abstract

Muñoz-Moreno et al. report that influenza A virus NS1 undergoes diverse and unpredictable evolutionary pathways based on its different phylogenetic lineages. A high-throughput approach using a barcoded library is used to test the interactions between NS1-recombinant viruses and to study their preference for specific or multiple hosts.

Original languageEnglish
Pages (from-to)3997-4009.e5
JournalCell Reports
Volume29
Issue number12
DOIs
Publication statusPublished - 2019 Dec 17

Keywords

  • barcoded library
  • IFN response
  • influenza virus
  • innate immunity
  • NS1 evolution
  • orthomyxovirus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Muñoz-Moreno, R., Martínez-Romero, C., Blanco-Melo, D., Forst, C. V., Nachbagauer, R., Benitez, A. A., Mena, I., Aslam, S., Balasubramaniam, V., Lee, I., Panis, M., Ayllón, J., Sachs, D., Park, M. S., Krammer, F., tenOever, B. R., & García-Sastre, A. (2019). Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses. Cell Reports, 29(12), 3997-4009.e5. https://doi.org/10.1016/j.celrep.2019.11.070