Viral genotypes and p53 expression in Epstein-Barr virus-associated primary malignant lymphomas of the intestines

Young Sik Kim, Jung Bun Kim, Yong Koo Kang, Eun Sook Nam, Seol Hee Park, Insun Kim

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    A small number (4% to 6%) of primary malignant lymphomas arising in the intestines express the EBV genome. However, in these tumors, the viral genotype and the role of tumor suppressor gene p53 have not been investigated. We sought to determine what genotype of EBV is frequently involved and whether the expression of p53 is related to these tumors. We used EBER-1 in situ hybridization and polymerase chain reactions (PCRs) for EBNA-1, EBNA-2A, and EBNA-2B to detect latent infection with EBV and to determine the genotype, respectively. In addition, we performed p53 PCR-SSCP (exons 5 through 9) and immunohistochemical analysis for p53. We found that EBV type B was present in 4 of 6 cases (67%); the genotype of the remaining cases could not be determined. The p53 PCR-SSCP indicated normal migration patterns in all malignant lymphomas, despite the fact that the tumor cells were strongly immunostained for p53 protein in 5 of the 6 cases. Thus, our study demonstrates that EBV-associated non-Hodgkin's lymphomas of the intestines in Korea are strongly related to the type B EBV and not to mutations of p53 gene. We suggest that EBV-associated intestinal lymphomas may arise through an interaction between the latent proteins of EBV and the wild-type p53 protein.

    Original languageEnglish
    Pages (from-to)1146-1152
    Number of pages7
    JournalHuman Pathology
    Volume30
    Issue number10
    DOIs
    Publication statusPublished - 1999 Oct

    Keywords

    • Epstein-Barr virus
    • Genotype
    • Intestine
    • Malignant lymphoma
    • P53

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine

    Fingerprint

    Dive into the research topics of 'Viral genotypes and p53 expression in Epstein-Barr virus-associated primary malignant lymphomas of the intestines'. Together they form a unique fingerprint.

    Cite this