Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Taegon Kim, Dongseok Moon, Jin Hyuk Park, Heehong Yang, Seongyeon Cho, Tai Hyun Park, Dong June Ahn

Research output: Contribution to journalArticle

Abstract

A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

Original languageEnglish
Pages (from-to)7582-7587
Number of pages6
JournalNanoscale
Volume11
Issue number16
DOIs
Publication statusPublished - 2019 Apr 23

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Odorant Receptors
Lipids
Fluorescence
Circular dichroism spectroscopy
Color
Phospholipids
Detergents
Micelles
Tryptophan
Quenching
Molecules
polydiacetylene
geraniol

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly. / Kim, Taegon; Moon, Dongseok; Park, Jin Hyuk; Yang, Heehong; Cho, Seongyeon; Park, Tai Hyun; Ahn, Dong June.

In: Nanoscale, Vol. 11, No. 16, 23.04.2019, p. 7582-7587.

Research output: Contribution to journalArticle

Kim, Taegon ; Moon, Dongseok ; Park, Jin Hyuk ; Yang, Heehong ; Cho, Seongyeon ; Park, Tai Hyun ; Ahn, Dong June. / Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly. In: Nanoscale. 2019 ; Vol. 11, No. 16. pp. 7582-7587.
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