Visualization of macrophage recruitment to inflammation lesions using highly sensitive and stable radionuclide-embedded gold nanoparticles as a nuclear bio-imaging platform

Sang Bong Lee, Ho Won Lee, Thoudam Debraj Singh, Yinghua Li, Sang Kyoon Kim, Sung Jin Cho, Sang Woo Lee, Shin Young Jeong, Byeong Cheol Ahn, Sangil Choi, In Kyu Lee, Dong-Kwon Lim, Jaetae Lee, Yong Hyun Jeon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Reliable and sensitive imaging tools are required to track macrophage migration and provide a better understating of their biological roles in various diseases. Here, we demonstrate the possibility of radioactive iodide-embedded gold nanoparticles (RIe-AuNPs) as a cell tracker for nuclear medicine imaging. To demonstrate this utility, we monitored macrophage migration to carrageenan-induced sites of acute inflammation in living subjects and visualized the effects of anti-inflammatory agents on this process. Macrophage labeling with RIe-AuNPs did not alter their biological functions such as cell proliferation, phenotype marker expression, or phagocytic activity.In vivo imaging with positron-emission tomography revealed the migration of labeled macrophages to carrageenan-induced inflammation lesions 3 h after transfer, with highest recruitment at 6 h and a slight decline of radioactive signal at 24 h; these findings were highly consistent with the data of a bio-distribution study. Treatment with dexamethasone (an anti-inflammation drug) or GSK5182 (an ERR? inverse agonist) hindered macrophage recruitment to the inflamed sites. Our findings suggest that a cell tracking strategy utilizing RIe-AuNPs will likely be highly useful in research related to macrophage-related disease and cell-based therapies.

Original languageEnglish
Pages (from-to)926-934
Number of pages9
JournalTheranostics
Volume7
Issue number4
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Radioisotopes
Gold
Nanoparticles
Macrophages
Inflammation
Carrageenan
Cell Tracking
Nuclear Medicine
Iodides
Cell- and Tissue-Based Therapy
Positron-Emission Tomography
Dexamethasone
Anti-Inflammatory Agents
Cell Proliferation
Phenotype
Research
Pharmaceutical Preparations

Keywords

  • Acute inflammation
  • Gold nanoparticles
  • Macrophage migration
  • Nuclear bio-imaging platform

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Cite this

Visualization of macrophage recruitment to inflammation lesions using highly sensitive and stable radionuclide-embedded gold nanoparticles as a nuclear bio-imaging platform. / Lee, Sang Bong; Lee, Ho Won; Singh, Thoudam Debraj; Li, Yinghua; Kim, Sang Kyoon; Cho, Sung Jin; Lee, Sang Woo; Jeong, Shin Young; Ahn, Byeong Cheol; Choi, Sangil; Lee, In Kyu; Lim, Dong-Kwon; Lee, Jaetae; Jeon, Yong Hyun.

In: Theranostics, Vol. 7, No. 4, 01.01.2017, p. 926-934.

Research output: Contribution to journalArticle

Lee, SB, Lee, HW, Singh, TD, Li, Y, Kim, SK, Cho, SJ, Lee, SW, Jeong, SY, Ahn, BC, Choi, S, Lee, IK, Lim, D-K, Lee, J & Jeon, YH 2017, 'Visualization of macrophage recruitment to inflammation lesions using highly sensitive and stable radionuclide-embedded gold nanoparticles as a nuclear bio-imaging platform', Theranostics, vol. 7, no. 4, pp. 926-934. https://doi.org/10.7150/thno.17131
Lee, Sang Bong ; Lee, Ho Won ; Singh, Thoudam Debraj ; Li, Yinghua ; Kim, Sang Kyoon ; Cho, Sung Jin ; Lee, Sang Woo ; Jeong, Shin Young ; Ahn, Byeong Cheol ; Choi, Sangil ; Lee, In Kyu ; Lim, Dong-Kwon ; Lee, Jaetae ; Jeon, Yong Hyun. / Visualization of macrophage recruitment to inflammation lesions using highly sensitive and stable radionuclide-embedded gold nanoparticles as a nuclear bio-imaging platform. In: Theranostics. 2017 ; Vol. 7, No. 4. pp. 926-934.
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