Visualization of myelination in GFP-transgenic zebrafish.

Seung Hyun Jung, Suhyun Kim, Ah Young Chung, Hyun Taek Kim, Ju Hoon So, Jaeho Ryu, Hae Chul Park, Cheol Hee Kim

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Abstract

The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis and hereditary myelin diseases. However, despite progress in understanding myelination regulation, many important questions remain unanswered. To investigate the mechanisms underlying myelination in vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of the mbp promoter. This transgenic fish displayed faithful EGFP expression in oligodendrocytes and Schwann cells in embryonic and adult zebrafish. Interestingly, although myelination progressed continuously in the postembryonic central nervous system, some of the spinal cord regions were filled with unmyelinated axons even in the adult spinal cord, suggesting functional differences between myelinated and unmyelinated axons. Our results suggest that this transgenic zebrafish could be a valuable animal model to study oligodendrocyte differentiation and myelination in vivo.

Original languageEnglish
Pages (from-to)592-597
Number of pages6
JournalDevelopmental dynamics : an official publication of the American Association of Anatomists
Volume239
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

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ASJC Scopus subject areas

  • Medicine(all)

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