Visualization of myelination in GFP-transgenic zebrafish

Seung Hyun Jung, Suhyun Kim, Ah Young Chung, Hyun Taek Kim, Ju Hoon So, Jaeho Ryu, Hae Chul Park, Cheol Hee Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis and hereditary myelin diseases. However, despite progress in understanding myelination regulation, many important questions remain unanswered. To investigate the mechanisms underlying myelination in vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of the mbp promoter. This transgenic fish displayed faithful EGFP expression in oligodendrocytes and Schwann cells in embryonic and adult zebrafish. Interestingly, although myelination progressed continuously in the postembryonic central nervous system, some of the spinal cord regions were filled with unmyelinated axons even in the adult spinal cord, suggesting functional differences between myelinated and unmyelinated axons. Our results suggest that this transgenic zebrafish could be a valuable animal model to study oligodendrocyte differentiation and myelination in vivo.

Original languageEnglish
Pages (from-to)598-609
Number of pages12
JournalDevelopmental Dynamics
Volume239
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Zebrafish
Axons
Oligodendroglia
Myelin Sheath
Spinal Cord
Inborn Genetic Diseases
Schwann Cells
Nervous System
Multiple Sclerosis
Vertebrates
Fishes
Central Nervous System
Animal Models
enhanced green fluorescent protein

Keywords

  • mbp
  • Myelination
  • Oligodendrocytes
  • Transgenic zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Jung, S. H., Kim, S., Chung, A. Y., Kim, H. T., So, J. H., Ryu, J., ... Kim, C. H. (2010). Visualization of myelination in GFP-transgenic zebrafish. Developmental Dynamics, 239(2), 598-609. https://doi.org/10.1002/dvdy.22163

Visualization of myelination in GFP-transgenic zebrafish. / Jung, Seung Hyun; Kim, Suhyun; Chung, Ah Young; Kim, Hyun Taek; So, Ju Hoon; Ryu, Jaeho; Park, Hae Chul; Kim, Cheol Hee.

In: Developmental Dynamics, Vol. 239, No. 2, 01.02.2010, p. 598-609.

Research output: Contribution to journalArticle

Jung, SH, Kim, S, Chung, AY, Kim, HT, So, JH, Ryu, J, Park, HC & Kim, CH 2010, 'Visualization of myelination in GFP-transgenic zebrafish', Developmental Dynamics, vol. 239, no. 2, pp. 598-609. https://doi.org/10.1002/dvdy.22163
Jung SH, Kim S, Chung AY, Kim HT, So JH, Ryu J et al. Visualization of myelination in GFP-transgenic zebrafish. Developmental Dynamics. 2010 Feb 1;239(2):598-609. https://doi.org/10.1002/dvdy.22163
Jung, Seung Hyun ; Kim, Suhyun ; Chung, Ah Young ; Kim, Hyun Taek ; So, Ju Hoon ; Ryu, Jaeho ; Park, Hae Chul ; Kim, Cheol Hee. / Visualization of myelination in GFP-transgenic zebrafish. In: Developmental Dynamics. 2010 ; Vol. 239, No. 2. pp. 598-609.
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