Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNA(Phe) with uridine-EDTA·Fe(II) at position 47

H. Han, P. B. Dervan

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

Original languageEnglish
Pages (from-to)4955-4959
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number11
DOIs
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • affinity cleaving
  • cleavage of RNA
  • yeast tRNA(Phe)

ASJC Scopus subject areas

  • General

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