Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47

Hogyu Han, Peter B. Dervan

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNAPhe in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

Original languageEnglish
Pages (from-to)4955-4959
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number11
Publication statusPublished - 1994 May 24
Externally publishedYes

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RNA, Transfer, Phe
Uridine
RNA
Dithiothreitol
Transfer RNA
Phenylalanine
Edetic Acid
Nucleotides
Yeasts
X-Rays
RNA primers

Keywords

  • Affinity cleaving
  • Cleavage of RNA
  • Yeast tRNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNAPhe in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.",
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T1 - Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage

T2 - Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47

AU - Han, Hogyu

AU - Dervan, Peter B.

PY - 1994/5/24

Y1 - 1994/5/24

N2 - To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNAPhe in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

AB - To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNAPhe in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

KW - Affinity cleaving

KW - Cleavage of RNA

KW - Yeast tRNA

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