Visualizing mitochondria and mouse intestine with a fluorescent complex of a naphthalene-based dipolar dye and serum albumin

Jong Min An, Heejo Moon, Yejin Kim, Sangrim Kang, Youngseo Kim, Yuna Jung, Sungnam Park, Peter Verwilst, B. Moon Kim, Jae Seung Kang, Dokyoung Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We have explored a new research field of fluorophores through the manipulation of fluorophore-binding proteins. The development of a new imaging agent for tracing a specific organelle or a particular site within a living organism has been of great interest in the field of basic science as well as translational medicine. In this work and for the first time, we will disclose a new naphthalene-based dipolar dye and its complex, with serum albumin (SA), and show their applicability for the selective imaging of mitochondria in cells and the intestine in a mouse. The SA-binding dipolar dye, IPNHC, was synthesized straightforwardly, and we identified its photophysical properties and binding mode with SA. IPNHC-SA complex showed a bright emission in the blue wavelength range with a high quantum yield and stability. In the fluorescence imaging study, bright fluorescence images of mouse intestines were observed under a UV light, as well as two-photon (TP) deep tissue imaging after intravenous injection of IPNHC and IPNHC-SA complex. The present findings hold great promise for the application of the fluorescent complex for use in the tracing and tracking of intestine-related diseases at clinical sites.

Original languageEnglish
Pages (from-to)7642-7651
Number of pages10
JournalJournal of Materials Chemistry B
Volume8
Issue number34
DOIs
Publication statusPublished - 2020 Sep 14

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Fingerprint Dive into the research topics of 'Visualizing mitochondria and mouse intestine with a fluorescent complex of a naphthalene-based dipolar dye and serum albumin'. Together they form a unique fingerprint.

Cite this