Abstract
The expression of vitamin D3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.
| Original language | English |
|---|---|
| Pages (from-to) | 235-247 |
| Number of pages | 13 |
| Journal | Immunology Letters |
| Volume | 86 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2003 May 1 |
| Externally published | Yes |
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Keywords
- FasL
- Melanoma cells
- NK cells
- ROS
- VDUP1
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Vitamin D3 up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species. / Song, Hyunkeun; Cho, Dae Ho; Jeon, Jun Ho; Han, Seung Hyun; Hur, Dae Young; Kim, Young Sang; Choi, Inpyo.
In: Immunology Letters, Vol. 86, No. 3, 01.05.2003, p. 235-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vitamin D3 up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species
AU - Song, Hyunkeun
AU - Cho, Dae Ho
AU - Jeon, Jun Ho
AU - Han, Seung Hyun
AU - Hur, Dae Young
AU - Kim, Young Sang
AU - Choi, Inpyo
PY - 2003/5/1
Y1 - 2003/5/1
N2 - The expression of vitamin D3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.
AB - The expression of vitamin D3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.
KW - FasL
KW - Melanoma cells
KW - NK cells
KW - ROS
KW - VDUP1
UR - http://www.scopus.com/inward/record.url?scp=0037403578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037403578&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(03)00024-5
DO - 10.1016/S0165-2478(03)00024-5
M3 - Article
C2 - 12706526
AN - SCOPUS:0037403578
VL - 86
SP - 235
EP - 247
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 3
ER -