Vitamin D3 up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species

Hyunkeun Song; Daeho Cho; Jun Ho Jeon; Seung Hyun Han; Dae Young Hur; Young Sang Kim; Inpyo Choi

doi:10.1016/S0165-2478(03)00024-5

Vitamin D₃ up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species

Hyunkeun Song, Daeho Cho, Jun Ho Jeon, Seung Hyun Han, Dae Young Hur, Young Sang Kim, Inpyo Choi

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The expression of vitamin D₃ up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D₃ (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.

Original language	English
Pages (from-to)	235-247
Number of pages	13
Journal	Immunology Letters
Volume	86
Issue number	3
DOIs	https://doi.org/10.1016/S0165-2478(03)00024-5
Publication status	Published - 2003 May 1
Externally published	Yes

Keywords

FasL
Melanoma cells
NK cells
ROS
VDUP1

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/S0165-2478(03)00024-5

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Vitamin D₃ up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species. / Song, Hyunkeun; Cho, Daeho; Jeon, Jun Ho; Han, Seung Hyun; Hur, Dae Young; Kim, Young Sang; Choi, Inpyo.

In: Immunology Letters, Vol. 86, No. 3, 01.05.2003, p. 235-247.

Research output: Contribution to journal › Article › peer-review

Song, Hyunkeun ; Cho, Daeho ; Jeon, Jun Ho ; Han, Seung Hyun ; Hur, Dae Young ; Kim, Young Sang ; Choi, Inpyo. / Vitamin D₃ up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species. In: Immunology Letters. 2003 ; Vol. 86, No. 3. pp. 235-247.

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abstract = "The expression of vitamin D3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.",

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