Vitamin E and gene expression in immune cells

Sung Nim Han, Oskar Adolfsson, Cheol Koo Lee, Tomas A. Prolla, Jose Ordovas, Simin Nikbin Meydani

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalAnnals of the New York Academy of Sciences
Volume1031
DOIs
Publication statusPublished - 2004
Externally publishedYes

Keywords

  • Aging
  • Gene expression
  • Immune cells
  • Vitamin E

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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  • Cite this

    Han, S. N., Adolfsson, O., Lee, C. K., Prolla, T. A., Ordovas, J., & Meydani, S. N. (2004). Vitamin E and gene expression in immune cells. Annals of the New York Academy of Sciences, 1031, 96-101. https://doi.org/10.1196/annals.1331.010