Vitamin E and gene expression in immune cells

Sung Nim Han, Oskar Adolfsson, Cheol-Koo Lee, Tomas A. Prolla, Jose Ordovas, Simin Nikbin Meydani

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalAnnals of the New York Academy of Sciences
Volume1031
DOIs
Publication statusPublished - 2004 Dec 1
Externally publishedYes

Fingerprint

T-cells
Vitamin E
Gene expression
T-Lymphocytes
Gene Expression
Genes
Aging of materials
Transcriptome
Signal transduction
Tocopherols
Microarray Analysis
Microarrays
Nutrition
Analysis of variance (ANOVA)
Cells
Vitamins
Inbred C57BL Mouse
Signal Transduction
Analysis of Variance
Cell Cycle

Keywords

  • Aging
  • Gene expression
  • Immune cells
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Han, S. N., Adolfsson, O., Lee, C-K., Prolla, T. A., Ordovas, J., & Meydani, S. N. (2004). Vitamin E and gene expression in immune cells. Annals of the New York Academy of Sciences, 1031, 96-101. https://doi.org/10.1196/annals.1331.010

Vitamin E and gene expression in immune cells. / Han, Sung Nim; Adolfsson, Oskar; Lee, Cheol-Koo; Prolla, Tomas A.; Ordovas, Jose; Meydani, Simin Nikbin.

In: Annals of the New York Academy of Sciences, Vol. 1031, 01.12.2004, p. 96-101.

Research output: Contribution to journalArticle

Han, SN, Adolfsson, O, Lee, C-K, Prolla, TA, Ordovas, J & Meydani, SN 2004, 'Vitamin E and gene expression in immune cells', Annals of the New York Academy of Sciences, vol. 1031, pp. 96-101. https://doi.org/10.1196/annals.1331.010
Han, Sung Nim ; Adolfsson, Oskar ; Lee, Cheol-Koo ; Prolla, Tomas A. ; Ordovas, Jose ; Meydani, Simin Nikbin. / Vitamin E and gene expression in immune cells. In: Annals of the New York Academy of Sciences. 2004 ; Vol. 1031. pp. 96-101.
@article{65325efa60ae47059b91bc63b682ac86,
title = "Vitamin E and gene expression in immune cells",
abstract = "Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.",
keywords = "Aging, Gene expression, Immune cells, Vitamin E",
author = "Han, {Sung Nim} and Oskar Adolfsson and Cheol-Koo Lee and Prolla, {Tomas A.} and Jose Ordovas and Meydani, {Simin Nikbin}",
year = "2004",
month = "12",
day = "1",
doi = "10.1196/annals.1331.010",
language = "English",
volume = "1031",
pages = "96--101",
journal = "Annals of The Lyceum of Natural History of New York",
issn = "0890-6564",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Vitamin E and gene expression in immune cells

AU - Han, Sung Nim

AU - Adolfsson, Oskar

AU - Lee, Cheol-Koo

AU - Prolla, Tomas A.

AU - Ordovas, Jose

AU - Meydani, Simin Nikbin

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.

AB - Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.

KW - Aging

KW - Gene expression

KW - Immune cells

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=14944355319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14944355319&partnerID=8YFLogxK

U2 - 10.1196/annals.1331.010

DO - 10.1196/annals.1331.010

M3 - Article

VL - 1031

SP - 96

EP - 101

JO - Annals of The Lyceum of Natural History of New York

JF - Annals of The Lyceum of Natural History of New York

SN - 0890-6564

ER -