Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells

Jung Eun Gil; Dong Hun Woo; Joong Hyun Shim; Sung Eun Kim; Hyun Ju You; Sung Hye Park; Sun Ha Paek; Suel Kee Kim; Jong Hoon Kim

doi:10.1016/j.febslet.2008.12.061

Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells

Jung Eun Gil, Dong Hun Woo, Joong Hyun Shim, Sung Eun Kim, Hyun Ju You, Sung Hye Park, Sun Ha Paek, Suel Kee Kim, Jong Hoon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.

Original language	English
Pages (from-to)	561-567
Number of pages	7
Journal	FEBS Letters
Volume	583
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2008.12.061
Publication status	Published - 2009 Feb 4

Keywords

Differentiation
Human embryonic stem cells
Oligodendrocytes
Vitronectin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.12.061

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title = "Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells",

abstract = "We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.",

keywords = "Differentiation, Human embryonic stem cells, Oligodendrocytes, Vitronectin",

author = "Gil, {Jung Eun} and Woo, {Dong Hun} and Shim, {Joong Hyun} and Kim, {Sung Eun} and You, {Hyun Ju} and Park, {Sung Hye} and Paek, {Sun Ha} and Kim, {Suel Kee} and Kim, {Jong Hoon}",

note = "Funding Information: This research was supported by the Seoul Research and Business Development Program (10574) and by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (R01-2006-000-11364-0).",

year = "2009",

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doi = "10.1016/j.febslet.2008.12.061",

language = "English",

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pages = "561--567",

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T1 - Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells

AU - Gil, Jung Eun

AU - Woo, Dong Hun

AU - Shim, Joong Hyun

AU - Kim, Sung Eun

AU - You, Hyun Ju

AU - Park, Sung Hye

AU - Paek, Sun Ha

AU - Kim, Suel Kee

AU - Kim, Jong Hoon

N1 - Funding Information: This research was supported by the Seoul Research and Business Development Program (10574) and by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (R01-2006-000-11364-0).

PY - 2009/2/4

Y1 - 2009/2/4

N2 - We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.

AB - We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.

KW - Differentiation

KW - Human embryonic stem cells

KW - Oligodendrocytes

KW - Vitronectin

UR - http://www.scopus.com/inward/record.url?scp=58649087788&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.12.061

DO - 10.1016/j.febslet.2008.12.061

M3 - Article

C2 - 19162023

AN - SCOPUS:58649087788

SN - 0014-5793

VL - 583

SP - 561

EP - 567

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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