Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells

Jung Eun Gil, Dong Hun Woo, Joong Hyun Shim, Sung Eun Kim, Hyun Ju You, Sung Hye Park, Sun Ha Paek, Suel Kee Kim, Jong Hoon Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.

Original languageEnglish
Pages (from-to)561-567
Number of pages7
JournalFEBS Letters
Volume583
Issue number3
DOIs
Publication statusPublished - 2009 Feb 4

Keywords

  • Differentiation
  • Human embryonic stem cells
  • Oligodendrocytes
  • Vitronectin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Gil, J. E., Woo, D. H., Shim, J. H., Kim, S. E., You, H. J., Park, S. H., Paek, S. H., Kim, S. K., & Kim, J. H. (2009). Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Letters, 583(3), 561-567. https://doi.org/10.1016/j.febslet.2008.12.061