Vocal fold wound healing after injection of human adipose-derived stem cells in a rabbit model

Seok Jin Hong, Sang Hyuk Lee, Sung Min Jin, Soon Young Kwon, Kwang Yoon Jung, Min Kyung Kim, Ho Park, Kyo Won Lee

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Conclusion: Injection of injured rabbit vocal folds with human adipose-derived stem cells (hADSCs) led to improved wound healing and fewer signs of scarring as demonstrated by a decreased collagen content in the treated folds compared with the untreated folds. hADSCs remained viable for up to 12 weeks in rabbit vocal folds. Objective: The aim of this study was to investigate the morphologic and histologic properties of scarred rabbit vocal folds following injection of hADSCs. Methods: This was a randomized, controlled animal study. Twenty-four vocal folds from 12 New Zealand rabbits were scarred using a CO2 laser and injected with either hADSCs (left vocal fold) or phosphate-buffered saline (right vocal fold). Every 4 weeks for the first 12 weeks after injection, an endoscopic examination was performed to assess the morphology of the vocal folds. Twelve weeks later the animals were euthanized and the tissues were stained for histology. Results: In comparison with the right vocal folds, there was significantly less granulation tissue in the hADSCs-injected left vocal folds (p < 0.05). Histological examination revealed excessive collagen deposition and perichondral fibrosis in the right vocal folds, whereas the left vocal folds exhibited better wound healing and less collagen deposition (p < 0.05). Among the 12 specimens injected with hADSCs, 4 specimens demonstrated viable hADSCs under immunofluorescent cytochemistry.

Original languageEnglish
Pages (from-to)1198-1204
Number of pages7
JournalActa Oto-Laryngologica
Issue number11
Publication statusPublished - 2011 Nov
Externally publishedYes


  • Adipose-derived stem cell transplantation
  • Scar
  • Vocal fold
  • Wound healing

ASJC Scopus subject areas

  • Otorhinolaryngology


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