Voltammetric discrimination of DNA single base-pair mismatches via Taq DNA ligase-based mismatch recognition

Jun Myung Kim, Chong Min Koo, Joohoon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An electrochemical approach for single base-specific detection of label-free target single-strand DNA (ssDNA) was demonstrated. The approach was based on the high ligation selectivity of Taq DNA ligase coupled with ferrocenated gold nanoparticle/streptavidin conjugate-based voltammetric detection method. The ferrocene (Fc) moieties of Fc-capped gold nanoparticle/streptavidin conjugates, which were selectively bound to the biotinylated DNA layer, were found to be close enough to the underlying electrode surface for facile electron transfer. The proposed electrochemical method was evaluated with label-free target DNA strands making all possible base pair combinations at the 3'-OH side of capture probe, and showed significant voltammetric signals only with a perfect match target strand.

Original languageEnglish
Pages (from-to)2019-2022
Number of pages4
JournalElectroanalysis
Volume23
Issue number9
DOIs
Publication statusPublished - 2011 Sep
Externally publishedYes

Keywords

  • Ferrocenated gold nanoparticle/streptavidin conjugates
  • Single base-pair mismatch
  • Taq DNA ligase
  • Voltammetric detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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