Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats

Hong Sik Lee, Lakshmi Bhagat, Jean Louis Frossard, Antti Hietaranta, Vijay P. Singh, Michael L. Steer, Ashok K. Saluja

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Abstract

Heat shock proteins (Hsps), induced by cell stress, are known to protect against cellular injury. Recent studies have indicated that Hsp60 expression, induced by exposure to water immersion stress, protects against pancreatitis induced by administration of supramaximal doses of cerulein in rats. However, the mechanisms responsible for this protection are not known. Methods: Rats were water-immersed for 3-12 hours. Pancreatitis was induced by cerulein administration. Results: The results confirm that prior induction of Hsp60 expression by water-immersion stress significantly ameliorates the severity of cerulein-induced pancreatitis as judged by the markedly reduced degree of hyperamylasemia, pancreatic edema, and acinar cell necrosis. Water immersion also prevents the subcellular redistribution of cathepsin B from a lysosome- enriched fraction to a heavier, zymogen granule-enriched fraction that is known to occur in this model of pancreatitis. Intra-acinar cell activation of trypsinogen that occurs shortly after exposure to a supramaximally stimulating dose of cerulein both in vivo and in vitro is prevented by prior water-immersion stress and Hsp60 expression. The protection against pancreatitis that follows water-immersion stress is not caused by alterations of cholecystokinin receptors, because water immersion does not alter the typical biphasic amylase secretory response to stimulation with cerulein. Conclusions: Water-immersion stress induces Hsp60 expression, ameliorates cerulein-induced pancreatitis, and prevents intra-acinar cell activation of trypsinogen. We suggest that Hsp60 protects against cerulein-induced pancreatitis by preventing trypsinogen activation within acinar cells.

Original languageEnglish
Pages (from-to)220-229
Number of pages10
JournalGastroenterology
Volume119
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1

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Chaperonin 60
Ceruletide
Immersion
Dehydration
Pancreatitis
Acinar Cells
Trypsinogen
Water
Hyperamylasemia
Cholecystokinin Receptors
Cathepsin B
Secretory Vesicles
Amylases
Heat-Shock Proteins
Lysosomes
Edema
Necrosis
Wounds and Injuries

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Lee, H. S., Bhagat, L., Frossard, J. L., Hietaranta, A., Singh, V. P., Steer, M. L., & Saluja, A. K. (2000). Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats. Gastroenterology, 119(1), 220-229. https://doi.org/10.1053/gast.2000.8551

Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats. / Lee, Hong Sik; Bhagat, Lakshmi; Frossard, Jean Louis; Hietaranta, Antti; Singh, Vijay P.; Steer, Michael L.; Saluja, Ashok K.

In: Gastroenterology, Vol. 119, No. 1, 01.01.2000, p. 220-229.

Research output: Contribution to journalArticle

Lee, HS, Bhagat, L, Frossard, JL, Hietaranta, A, Singh, VP, Steer, ML & Saluja, AK 2000, 'Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats', Gastroenterology, vol. 119, no. 1, pp. 220-229. https://doi.org/10.1053/gast.2000.8551
Lee, Hong Sik ; Bhagat, Lakshmi ; Frossard, Jean Louis ; Hietaranta, Antti ; Singh, Vijay P. ; Steer, Michael L. ; Saluja, Ashok K. / Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats. In: Gastroenterology. 2000 ; Vol. 119, No. 1. pp. 220-229.
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