Water-soluble conjugated polyelectrolytes with molecular bumper for efficient FRET biosensor

Han Young Woo, Doojin Vak, Guillermo C. Bazan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the design, synthesis and photophysical properties of two new water-soluble conjugated polyelectrolytes, poly(9,9' ?bis(6-NNN- trimethylammoniumhexyl)fluorene-alt-1,4-(2,5-bis(6-N,N,N- trimethylammoniumhexyloxy))phenylene) tetrabromide (P1i) and poly((10,10'-bis(6-N,N,N-trimethylammoniumhexyl)-10H-spiro(anthracene-9, 9'-fluorene))-alt1, 4-(2,5-bis(6-N,N,N-trimethylammoniumhexyloxy))phenylene) tetrabromide (P2i). They have same electronic conjugation but the main structural difference is the presence of the anthracenyl substituent orthogonal to the polymer main backbone in P2i. Fluorescence resonance energy transfer (FRET) from the two polymers as FRET donors to fluorescein-labeled single stranded DNA (ssDNA·Fl) was studied. It is observed the emission from ssDNA-Fl via FRET by excitation of P2i with a FRET efficiency of -60%. Fluorescein is not emissive within the ssDNA-Fl/Pli complex. We also observed clear PL quenching (Φ = 0.8 → Φ = 0.27) of fluorescein for P2i after electrostatic complexation with ssDNA-Fl. It suggests a quenching pathway of E emission through photo-induced charge transfer (PCT) in the electrostatic complex. Both P1i and P2i have same HOMO-LUMO electronic structures and a similar thermodynamic driving force for either FRET or PCT. It appears that PCT operates to a larger extent with P1i, proposing that the presence of the "molecular bumper" in P2i increases Fl emission by increasing the donor-acceptor distance, which decreases more acutely PCT quenching, relative to FRET.

Original languageEnglish
Title of host publicationOrganic Electronics
Subtitle of host publicationMaterials, Devices and Applications
Pages40-45
Number of pages6
Publication statusPublished - 2006 Dec 1
Externally publishedYes
Event2006 MRS Fall Meeting - Boston, MA, United States
Duration: 2006 Nov 272006 Dec 1

Publication series

NameMaterials Research Society Symposium Proceedings
Volume965
ISSN (Print)0272-9172

Other

Other2006 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period06/11/2706/12/1

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Woo, H. Y., Vak, D., & Bazan, G. C. (2006). Water-soluble conjugated polyelectrolytes with molecular bumper for efficient FRET biosensor. In Organic Electronics: Materials, Devices and Applications (pp. 40-45). (Materials Research Society Symposium Proceedings; Vol. 965).