Water-soluble polyelectrolytes for FRET-based DNA detection

Han Young Woo, Okhil Kumar Nag, Jinseck Kim, Mijeong Kang, Guillermo C. Bazan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.

Original languageEnglish
JournalMolecular Crystals and Liquid Crystals
Volume486
DOIs
Publication statusPublished - 2008 Jul 28
Externally publishedYes

Fingerprint

resonance fluorescence
Polyelectrolytes
DNA
deoxyribonucleic acid
bumpers
energy transfer
Water
Quenching
water
Single-Stranded DNA
DNA sequences
quenching
Fluorescein
Energy transfer
Electronic structure
Electrostatics
Assays
Polymers
Fluorescence
spacers

Keywords

  • Biosensor
  • Conjugated polyelectrolyte
  • DNA detection
  • DNA sensor
  • FRET

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Water-soluble polyelectrolytes for FRET-based DNA detection. / Woo, Han Young; Nag, Okhil Kumar; Kim, Jinseck; Kang, Mijeong; Bazan, Guillermo C.

In: Molecular Crystals and Liquid Crystals, Vol. 486, 28.07.2008.

Research output: Contribution to journalArticle

Woo, Han Young ; Nag, Okhil Kumar ; Kim, Jinseck ; Kang, Mijeong ; Bazan, Guillermo C. / Water-soluble polyelectrolytes for FRET-based DNA detection. In: Molecular Crystals and Liquid Crystals. 2008 ; Vol. 486.
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