Water-soluble polyelectrolytes for FRET-based DNA detection

Han Young Woo, Okhil Kumar Nag, Jinseck Kim, Mijeong Kang, Guillermo C. Bazan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.

Original languageEnglish
Pages (from-to)244/[1286]-249/[1291]
JournalMolecular Crystals and Liquid Crystals
Volume486
DOIs
Publication statusPublished - 2008

Keywords

  • Biosensor
  • Conjugated polyelectrolyte
  • DNA detection
  • DNA sensor
  • FRET

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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