TY - JOUR
T1 - WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells
AU - Kho, Yoonjung
AU - Kim, Sungchan
AU - Yoon, Byung Sun
AU - Moon, Jai Hee
AU - Kwak, Sungwook
AU - Park, Gyuman
AU - Woo, Junghee
AU - Oh, Sejong
AU - Hong, Kichang
AU - Kim, Saehun
AU - Kim, Hyunggee
AU - You, Seungkwon
AU - Choi, Yunjaie
PY - 2008/4
Y1 - 2008/4
N2 - In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.
AB - In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.
KW - Apoptosis
KW - Mammary epithelial cell
KW - NFκB
KW - TNFα
KW - WDNM1
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U2 - 10.1080/10495390801887361
DO - 10.1080/10495390801887361
M3 - Article
C2 - 18432400
AN - SCOPUS:42649088130
VL - 19
SP - 89
EP - 103
JO - Animal Biotechnology
JF - Animal Biotechnology
SN - 1049-5398
IS - 2
ER -