Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel

Myounggun Jeon, Hey Jin Tak, Yun Kyung Kim, Eui Sung Yoon, Sehyun Shin, Il Joo Cho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a new well-based microfluidic cell culture chip for monitoring non-contacting cell-to-cell interactions through micro-channels between chambers. The chip is fabricated with PDMS layers using SU-8 as molds. The fabricated chip was applied to monitor non-contacting cell-to-cell interaction between human glioblastoma cells and tau-engineered cells to study the association between malignant brain tumor and Alzheimer's disease (AD). Our results clearly demonstrated that the secreted components from glioblastoma cells enhanced tau aggregation. We have successfully demonstrated that the proposed well-based cell-to-cell interaction-monitoring chip can make two different cells interact through micro-channels without contacting each other using interactions between tau-BiFC cells and glioblastoma cells.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages418-421
Number of pages4
DOIs
Publication statusPublished - 2013 Dec 1
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 2013 Jun 162013 Jun 20

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CountrySpain
CityBarcelona
Period13/6/1613/6/20

Fingerprint

Microchannels
Microfluidics
Monitoring
Molds
Cell culture
Tumors
Brain
Agglomeration

Keywords

  • Cell to cell interaction
  • glioblastoma cell
  • microfluidic chip
  • non-contacting
  • PDMS molding
  • SU-8 mold
  • tau aggregation

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Jeon, M., Tak, H. J., Kim, Y. K., Yoon, E. S., Shin, S., & Cho, I. J. (2013). Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 418-421). [6626792] https://doi.org/10.1109/Transducers.2013.6626792

Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel. / Jeon, Myounggun; Tak, Hey Jin; Kim, Yun Kyung; Yoon, Eui Sung; Shin, Sehyun; Cho, Il Joo.

2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 418-421 6626792.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jeon, M, Tak, HJ, Kim, YK, Yoon, ES, Shin, S & Cho, IJ 2013, Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626792, pp. 418-421, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 13/6/16. https://doi.org/10.1109/Transducers.2013.6626792
Jeon M, Tak HJ, Kim YK, Yoon ES, Shin S, Cho IJ. Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 418-421. 6626792 https://doi.org/10.1109/Transducers.2013.6626792
Jeon, Myounggun ; Tak, Hey Jin ; Kim, Yun Kyung ; Yoon, Eui Sung ; Shin, Sehyun ; Cho, Il Joo. / Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 418-421
@inproceedings{961e9dc4bc47416aae9cdb4c5533258d,
title = "Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel",
abstract = "We present a new well-based microfluidic cell culture chip for monitoring non-contacting cell-to-cell interactions through micro-channels between chambers. The chip is fabricated with PDMS layers using SU-8 as molds. The fabricated chip was applied to monitor non-contacting cell-to-cell interaction between human glioblastoma cells and tau-engineered cells to study the association between malignant brain tumor and Alzheimer's disease (AD). Our results clearly demonstrated that the secreted components from glioblastoma cells enhanced tau aggregation. We have successfully demonstrated that the proposed well-based cell-to-cell interaction-monitoring chip can make two different cells interact through micro-channels without contacting each other using interactions between tau-BiFC cells and glioblastoma cells.",
keywords = "Cell to cell interaction, glioblastoma cell, microfluidic chip, non-contacting, PDMS molding, SU-8 mold, tau aggregation",
author = "Myounggun Jeon and Tak, {Hey Jin} and Kim, {Yun Kyung} and Yoon, {Eui Sung} and Sehyun Shin and Cho, {Il Joo}",
year = "2013",
month = "12",
day = "1",
doi = "10.1109/Transducers.2013.6626792",
language = "English",
isbn = "9781467359818",
pages = "418--421",
booktitle = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",

}

TY - GEN

T1 - Well-based microfluidic chip for monitoring non-contacting cell-to-cell interactions through microchannel

AU - Jeon, Myounggun

AU - Tak, Hey Jin

AU - Kim, Yun Kyung

AU - Yoon, Eui Sung

AU - Shin, Sehyun

AU - Cho, Il Joo

PY - 2013/12/1

Y1 - 2013/12/1

N2 - We present a new well-based microfluidic cell culture chip for monitoring non-contacting cell-to-cell interactions through micro-channels between chambers. The chip is fabricated with PDMS layers using SU-8 as molds. The fabricated chip was applied to monitor non-contacting cell-to-cell interaction between human glioblastoma cells and tau-engineered cells to study the association between malignant brain tumor and Alzheimer's disease (AD). Our results clearly demonstrated that the secreted components from glioblastoma cells enhanced tau aggregation. We have successfully demonstrated that the proposed well-based cell-to-cell interaction-monitoring chip can make two different cells interact through micro-channels without contacting each other using interactions between tau-BiFC cells and glioblastoma cells.

AB - We present a new well-based microfluidic cell culture chip for monitoring non-contacting cell-to-cell interactions through micro-channels between chambers. The chip is fabricated with PDMS layers using SU-8 as molds. The fabricated chip was applied to monitor non-contacting cell-to-cell interaction between human glioblastoma cells and tau-engineered cells to study the association between malignant brain tumor and Alzheimer's disease (AD). Our results clearly demonstrated that the secreted components from glioblastoma cells enhanced tau aggregation. We have successfully demonstrated that the proposed well-based cell-to-cell interaction-monitoring chip can make two different cells interact through micro-channels without contacting each other using interactions between tau-BiFC cells and glioblastoma cells.

KW - Cell to cell interaction

KW - glioblastoma cell

KW - microfluidic chip

KW - non-contacting

KW - PDMS molding

KW - SU-8 mold

KW - tau aggregation

UR - http://www.scopus.com/inward/record.url?scp=84891697299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891697299&partnerID=8YFLogxK

U2 - 10.1109/Transducers.2013.6626792

DO - 10.1109/Transducers.2013.6626792

M3 - Conference contribution

AN - SCOPUS:84891697299

SN - 9781467359818

SP - 418

EP - 421

BT - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

ER -