Wide-field reflection phase microscope

Zahid Yaqoob, Toyohiko Yamauchi, Dan Fu, Wonshik Choi, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Wide-field reflection phase microscopy is highly desired for depth-resolved measurement of cellular structures without the need for raster scanning. We report a low coherence reflection phase microscope based on time-domain optical coherence tomography and off-axis interferometry. The setup uniquely provides the desired angular shift to the reference beam for off-axis interferometry while promising equal path length across the whole reference beam. We show subnanometer path-length sensitivity of our instrument and demonstrate high-speed imaging of membrane fluctuations in eukaryotic cells.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7904
DOIs
Publication statusPublished - 2011 Apr 29
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII - San Francisco, CA, United States
Duration: 2011 Jan 242011 Jan 27

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
CountryUnited States
CitySan Francisco, CA
Period11/1/2411/1/27

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Keywords

  • Low coherence interferometry
  • Optical coherence tomography
  • Quantitative phase imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yaqoob, Z., Yamauchi, T., Fu, D., Choi, W., Dasari, R. R., & Feld, M. S. (2011). Wide-field reflection phase microscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7904). [79040T] https://doi.org/10.1117/12.875877