Wide-field reflection phase microscope

Zahid Yaqoob, Toyohiko Yamauchi, Dan Fu, Wonshik Choi, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Wide-field reflection phase microscopy is highly desired for depth-resolved measurement of cellular structures without the need for raster scanning. We report a low coherence reflection phase microscope based on time-domain optical coherence tomography and off-axis interferometry. The setup uniquely provides the desired angular shift to the reference beam for off-axis interferometry while promising equal path length across the whole reference beam. We show subnanometer path-length sensitivity of our instrument and demonstrate high-speed imaging of membrane fluctuations in eukaryotic cells.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7904
DOIs
Publication statusPublished - 2011 Apr 29
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII - San Francisco, CA, United States
Duration: 2011 Jan 242011 Jan 27

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
CountryUnited States
CitySan Francisco, CA
Period11/1/2411/1/27

Fingerprint

Interferometry
interferometry
Microscopes
microscopes
raster scanning
depth measurement
Optical tomography
Optical Coherence Tomography
Eukaryotic Cells
Cellular Structures
Microscopy
Microscopic examination
tomography
high speed
membranes
microscopy
Membranes
Scanning
Imaging techniques
sensitivity

Keywords

  • Low coherence interferometry
  • Optical coherence tomography
  • Quantitative phase imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yaqoob, Z., Yamauchi, T., Fu, D., Choi, W., Dasari, R. R., & Feld, M. S. (2011). Wide-field reflection phase microscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7904). [79040T] https://doi.org/10.1117/12.875877

Wide-field reflection phase microscope. / Yaqoob, Zahid; Yamauchi, Toyohiko; Fu, Dan; Choi, Wonshik; Dasari, Ramachandra R.; Feld, Michael S.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7904 2011. 79040T.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yaqoob, Z, Yamauchi, T, Fu, D, Choi, W, Dasari, RR & Feld, MS 2011, Wide-field reflection phase microscope. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7904, 79040T, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, San Francisco, CA, United States, 11/1/24. https://doi.org/10.1117/12.875877
Yaqoob Z, Yamauchi T, Fu D, Choi W, Dasari RR, Feld MS. Wide-field reflection phase microscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7904. 2011. 79040T https://doi.org/10.1117/12.875877
Yaqoob, Zahid ; Yamauchi, Toyohiko ; Fu, Dan ; Choi, Wonshik ; Dasari, Ramachandra R. ; Feld, Michael S. / Wide-field reflection phase microscope. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7904 2011.
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