Abstract
A phytochemical study to obtain new nitric oxide (NO) inhibitors resulted in the isolation of five new withanolides from the whole plants of Physalis peruviana. The structures were determined on the basis of extensive NMR spectroscopic data analysis as well as the time-dependent density functional theory (TDDFT) electronic circular dichroism (ECD) calculations. The NO inhibitory effects were examined by inhibiting NO release in lipopolysaccharide-stimulated murine microglial BV-2 cells. Molecular docking studies showed the strong interactions of bioactive compounds with the inducible nitric oxide synthase (iNOS) protein, revealing the potential mechanism of NO inhibition of bioactive compounds.
Original language | English |
---|---|
Pages (from-to) | 585-593 |
Number of pages | 9 |
Journal | Bioorganic Chemistry |
Volume | 87 |
DOIs | |
Publication status | Published - 2019 Jun |
Keywords
- Molecular docking
- NO inhibitors
- Physalis peruviana
- Withanolides
- iNOS
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Drug Discovery
- Organic Chemistry