Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon, Mi Hyun Choi, Ja-Hyun Baik

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [ 3 H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull-down, and competition-binding assays used in the analysis of the D2R-Wnt5a interaction.

    Original languageEnglish
    Title of host publicationDopamine Receptor Technologies
    PublisherSpringer New York
    Pages65-78
    Number of pages14
    ISBN (Print)9781493921966, 9781493921959
    DOIs
    Publication statusPublished - 2014 Dec 4

    Keywords

    • Co-immunoprecipitation
    • Dopamine D2 receptor
    • GST pull-down assay
    • Radioligand binding
    • Wnt5a

    ASJC Scopus subject areas

    • Medicine(all)
    • Neuroscience(all)

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