Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity

Hua Zi Piao, In Young Choi, Jin Sun Park, Hee Sun Kim, Jae Hoon Cheong, Kun Ho Son, Su Jin Jeon, Kwang Ho Ko, Won-Ki Kim

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Previously, we and others have demonstrated that wogonin, an active component from the root of Scutellaria baicalensis Georgi, has a neuroprotective effect in cerebral ischemic insult. The neuroprotective effect of wogonin may at least in part be due to its anti-inflammatory properties. Microglial cells, well-known residential macrophages in the central nervous system, migrate to the ischemic lesion and play a pivotal role in the development of chronic inflammation. In the present study, we observed that wogonin potently inhibited microglial migration toward a chemokine, monocyte chemoattractant protein-1 (MCP-1). The anti-migratory effect of wogonin was provoked at nanomolar concentrations, at which wogonin did not significantly inhibit the production of cytokines and chemokines. NF-κB has previously shown to regulate microglial cell migration, and activation of cAMP-signaling pathway has also been associated with inhibition of microglial cell motility. In the present study, wogonin at low micromolar concentrations completely suppressed the activity of NF-κB in MCP-1-stimulated microglia, and NF-κB inhibitors such as N-acetyl cysteine and pyrrolidinedithiocarbamate inhibited the MCP-1-induced migration of microglial cells. However, wogonin did not stimulate the production of cAMP in microglial cells. Our results indicate that the anti-inflammatory activity of wogonin is exerted at least in part by suppressing microglial cell motility via inhibition of NF-κB activity.

Original languageEnglish
Pages (from-to)1658-1662
Number of pages5
JournalInternational Immunopharmacology
Volume8
Issue number12
DOIs
Publication statusPublished - 2008 Dec 10
Externally publishedYes

    Fingerprint

Keywords

  • cAMP
  • MCP-1
  • Microglia
  • Migration
  • NF-κB
  • Wogonin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Piao, H. Z., Choi, I. Y., Park, J. S., Kim, H. S., Cheong, J. H., Son, K. H., Jeon, S. J., Ko, K. H., & Kim, W-K. (2008). Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity. International Immunopharmacology, 8(12), 1658-1662. https://doi.org/10.1016/j.intimp.2008.07.018