Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity

Hua Zi Piao, In Young Choi, Jin Sun Park, Hee Sun Kim, Jae Hoon Cheong, Kun Ho Son, Su Jin Jeon, Kwang Ho Ko, Won-Ki Kim

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Previously, we and others have demonstrated that wogonin, an active component from the root of Scutellaria baicalensis Georgi, has a neuroprotective effect in cerebral ischemic insult. The neuroprotective effect of wogonin may at least in part be due to its anti-inflammatory properties. Microglial cells, well-known residential macrophages in the central nervous system, migrate to the ischemic lesion and play a pivotal role in the development of chronic inflammation. In the present study, we observed that wogonin potently inhibited microglial migration toward a chemokine, monocyte chemoattractant protein-1 (MCP-1). The anti-migratory effect of wogonin was provoked at nanomolar concentrations, at which wogonin did not significantly inhibit the production of cytokines and chemokines. NF-κB has previously shown to regulate microglial cell migration, and activation of cAMP-signaling pathway has also been associated with inhibition of microglial cell motility. In the present study, wogonin at low micromolar concentrations completely suppressed the activity of NF-κB in MCP-1-stimulated microglia, and NF-κB inhibitors such as N-acetyl cysteine and pyrrolidinedithiocarbamate inhibited the MCP-1-induced migration of microglial cells. However, wogonin did not stimulate the production of cAMP in microglial cells. Our results indicate that the anti-inflammatory activity of wogonin is exerted at least in part by suppressing microglial cell motility via inhibition of NF-κB activity.

Original languageEnglish
Pages (from-to)1658-1662
Number of pages5
JournalInternational Immunopharmacology
Volume8
Issue number12
DOIs
Publication statusPublished - 2008 Dec 10
Externally publishedYes

Fingerprint

NF-kappa B
Cell Movement
Chemokine CCL2
Neuroprotective Agents
Chemokines
Anti-Inflammatory Agents
Scutellaria baicalensis
wogonin
Microglia
Cysteine
Central Nervous System
Macrophages
Cytokines
Inflammation

Keywords

  • cAMP
  • MCP-1
  • Microglia
  • Migration
  • NF-κB
  • Wogonin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity. / Piao, Hua Zi; Choi, In Young; Park, Jin Sun; Kim, Hee Sun; Cheong, Jae Hoon; Son, Kun Ho; Jeon, Su Jin; Ko, Kwang Ho; Kim, Won-Ki.

In: International Immunopharmacology, Vol. 8, No. 12, 10.12.2008, p. 1658-1662.

Research output: Contribution to journalArticle

Piao, HZ, Choi, IY, Park, JS, Kim, HS, Cheong, JH, Son, KH, Jeon, SJ, Ko, KH & Kim, W-K 2008, 'Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity', International Immunopharmacology, vol. 8, no. 12, pp. 1658-1662. https://doi.org/10.1016/j.intimp.2008.07.018
Piao, Hua Zi ; Choi, In Young ; Park, Jin Sun ; Kim, Hee Sun ; Cheong, Jae Hoon ; Son, Kun Ho ; Jeon, Su Jin ; Ko, Kwang Ho ; Kim, Won-Ki. / Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity. In: International Immunopharmacology. 2008 ; Vol. 8, No. 12. pp. 1658-1662.
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