XIAP reverses various functional activities of FRNK in endothelial cells

Sunyoung Ahn, Hyun Jeong Kim, Sung-Gil Chi, Heonyong Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In endothelial cells, focal adhesion kinase (FAK) regulates cell proliferation, migration, adhesion, and shear-stimulated activation of MAPK. We recently found that FAK is recruited into focal adhesion (FA) sites through interactions with XIAP (X-chromosome linked inhibitor of apoptosis protein) and activated by Src kinase in response to shear stress. In this study, we examined which domain(s) of FAK is(are) important for various vascular functions such as FA recruiting, XIAP-binding and shear stress-stimulated ERK activation. Through a series of experiments, we determined that the FRNK domain is recruited into FA sites and promotes endothelial cell adhesion. Interestingly, XIAP knockdown was shown to reduce FA recruitment of FRNK and the cell adhesive effect of FRNK. In addition, we found that XIAP interacts with FRNK, suggesting cross-talk between XIAP and FRNK. We also demonstrated that FRNK inhibits endothelial cell migration and shear-stimulated ERK activation. These inhibitory effects of FRNK were reversed by XIAP knockdown. Taken together, we can conclude that XIAP plays a key role in vascular functions of FRNK or FRNK domain-mediated vascular functions of FAK.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number2
DOIs
Publication statusPublished - 2012 Mar 9

Fingerprint

X-Linked Inhibitor of Apoptosis Protein
Inhibitor of Apoptosis Proteins
Endothelial cells
X Chromosome
Chromosomes
Endothelial Cells
Focal Adhesion Protein-Tyrosine Kinases
Focal Adhesions
Adhesion
Blood Vessels
Chemical activation
Cell Adhesion
Cell Movement
Shear stress
src-Family Kinases
Cell adhesion
Cell proliferation
Protein Binding
Adhesives
Cell Proliferation

Keywords

  • Endothelial cells
  • FAK
  • Focal adhesions
  • FRNK
  • XIAP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

XIAP reverses various functional activities of FRNK in endothelial cells. / Ahn, Sunyoung; Kim, Hyun Jeong; Chi, Sung-Gil; Park, Heonyong.

In: Biochemical and Biophysical Research Communications, Vol. 419, No. 2, 09.03.2012, p. 419-424.

Research output: Contribution to journalArticle

Ahn, Sunyoung ; Kim, Hyun Jeong ; Chi, Sung-Gil ; Park, Heonyong. / XIAP reverses various functional activities of FRNK in endothelial cells. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 419, No. 2. pp. 419-424.
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