Xylanase production in solid state fermentation by Aspergillus niger mutant using statistical experimental designs

Y. Park, S. Kang, J. Lee, S. Hong, S. Kim

Research output: Contribution to journalArticlepeer-review

152 Citations (Scopus)

Abstract

The initial moisture content, cultivation time, inoculum size and concentration of basal medium were optimized in solid state fermentation (SSF) for the production of xylanase by an Aspergillus niger mutant using statistical experimental designs. The cultivation time and concentration of basal medium were the most important factors affecting xylanase activity. An inoculum size of 5×105 spores/g, initial moisture content of 65%, cultivation time of 5 days and 10 times concentration of basal medium containing 50 times concentration of corn steep liquor were optimum for xylanase production in SSF. Under the optimized conditions, the activity and productivity of xylanase obtained after 5 days of fermentation were 5,071 IU/g of rice straw and 14,790 IU l-1 h-1, respectively. The xylanase activity predicted by a polynomial model was 5,484 IU/g of rice straw.

Original languageEnglish
Pages (from-to)761-766
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume58
Issue number6
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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