YAP and TAZ regulate skin wound healing

Min Jung Lee, Mi Ran Byun, Makoto Furutani-Seiki, Jeong Ho Hong, Han Sung Jung

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The Hippo signaling pathway regulates organ size, tissue regeneration, and stem cell self-renewal. The two key downstream transcription coactivators in this pathway, Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), mediate the major gene regulation and biological functions of the Hippo pathway. The biological functions of YAP and TAZ in many tissues are known; however, their roles in skin wound healing remain unclear. To analyze whether YAP and/or TAZ are required for cutaneous wound healing, we performed small interfering RNA (siRNA)-mediated knockdown of YAP/TAZ in full-thickness skin wounds. YAP is strongly expressed in the nucleus and cytoplasm in the epidermis and hair follicle. Interestingly, YAP is expressed in the nucleus in the dermis at 2 and 7 days after wounding. TAZ normally localizes to the cytoplasm in the dermis but is distributed in both the nucleus and cytoplasm at 1 day after wounding. The knockdown of YAP and TAZ markedly delayed the rate of wound closure and reduced the transforming growth factor-β1 (TGF-β1) expression in the wound. YAP and TAZ also modulate the expression of TGF-β1 signaling pathway components such as Smad-2, p21, and Smad-7. These results suggest that YAP and TAZ localization to the nucleus is required for skin wound healing.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalJournal of Investigative Dermatology
Volume134
Issue number2
DOIs
Publication statusPublished - 2014 Feb

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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    Lee, M. J., Byun, M. R., Furutani-Seiki, M., Hong, J. H., & Jung, H. S. (2014). YAP and TAZ regulate skin wound healing. Journal of Investigative Dermatology, 134(2), 518-525. https://doi.org/10.1038/jid.2013.339