YAP and TAZ regulate skin wound healing

Min Jung Lee, Mi Ran Byun, Makoto Furutani-Seiki, Jeong-Ho Hong, Han Sung Jung

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The Hippo signaling pathway regulates organ size, tissue regeneration, and stem cell self-renewal. The two key downstream transcription coactivators in this pathway, Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), mediate the major gene regulation and biological functions of the Hippo pathway. The biological functions of YAP and TAZ in many tissues are known; however, their roles in skin wound healing remain unclear. To analyze whether YAP and/or TAZ are required for cutaneous wound healing, we performed small interfering RNA (siRNA)-mediated knockdown of YAP/TAZ in full-thickness skin wounds. YAP is strongly expressed in the nucleus and cytoplasm in the epidermis and hair follicle. Interestingly, YAP is expressed in the nucleus in the dermis at 2 and 7 days after wounding. TAZ normally localizes to the cytoplasm in the dermis but is distributed in both the nucleus and cytoplasm at 1 day after wounding. The knockdown of YAP and TAZ markedly delayed the rate of wound closure and reduced the transforming growth factor-β1 (TGF-β1) expression in the wound. YAP and TAZ also modulate the expression of TGF-β1 signaling pathway components such as Smad-2, p21, and Smad-7. These results suggest that YAP and TAZ localization to the nucleus is required for skin wound healing.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalJournal of Investigative Dermatology
Volume134
Issue number2
DOIs
Publication statusPublished - 2014 Feb 1

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Wound Healing
Skin
Proteins
Cytoplasm
Transforming Growth Factors
Dermis
Wounds and Injuries
Tissue regeneration
Organ Size
Hair Follicle
Transcription
Stem cells
Gene expression
Epidermis
Small Interfering RNA
Regeneration
Tissue
Genes

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Lee, M. J., Byun, M. R., Furutani-Seiki, M., Hong, J-H., & Jung, H. S. (2014). YAP and TAZ regulate skin wound healing. Journal of Investigative Dermatology, 134(2), 518-525. https://doi.org/10.1038/jid.2013.339

YAP and TAZ regulate skin wound healing. / Lee, Min Jung; Byun, Mi Ran; Furutani-Seiki, Makoto; Hong, Jeong-Ho; Jung, Han Sung.

In: Journal of Investigative Dermatology, Vol. 134, No. 2, 01.02.2014, p. 518-525.

Research output: Contribution to journalArticle

Lee, MJ, Byun, MR, Furutani-Seiki, M, Hong, J-H & Jung, HS 2014, 'YAP and TAZ regulate skin wound healing', Journal of Investigative Dermatology, vol. 134, no. 2, pp. 518-525. https://doi.org/10.1038/jid.2013.339
Lee, Min Jung ; Byun, Mi Ran ; Furutani-Seiki, Makoto ; Hong, Jeong-Ho ; Jung, Han Sung. / YAP and TAZ regulate skin wound healing. In: Journal of Investigative Dermatology. 2014 ; Vol. 134, No. 2. pp. 518-525.
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