Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA

Ki Moon Seong, Sang Oun Jung, Hag Dong Kim, Hee Ju Kim, You Jin Jung, Soo Young Choi, Joon Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.

Original languageEnglish
Pages (from-to)356-361
Number of pages6
JournalFEBS Letters
Volume586
Issue number4
DOIs
Publication statusPublished - 2012 Feb 17

Fingerprint

Lyases
Fungal Proteins
DNA Repair
Yeast
Yeasts
Pyrimidine Dimers
Repair
DNA
Protein Biosynthesis
Substrate Specificity
Escherichia coli
Mutation
Enzyme activity
Substrates
Enzymes
Catalyst activity
ribosomal protein S3
Proteins
8-hydroxyguanine

Keywords

  • 8-Oxo-G
  • AP sites
  • DNA repair
  • Lyase
  • Pyrimidine dimer
  • Yeast Rps3p

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Seong, K. M., Jung, S. O., Kim, H. D., Kim, H. J., Jung, Y. J., Choi, S. Y., & Kim, J. (2012). Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA. FEBS Letters, 586(4), 356-361. https://doi.org/10.1016/j.febslet.2011.12.030

Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA. / Seong, Ki Moon; Jung, Sang Oun; Kim, Hag Dong; Kim, Hee Ju; Jung, You Jin; Choi, Soo Young; Kim, Joon.

In: FEBS Letters, Vol. 586, No. 4, 17.02.2012, p. 356-361.

Research output: Contribution to journalArticle

Seong, KM, Jung, SO, Kim, HD, Kim, HJ, Jung, YJ, Choi, SY & Kim, J 2012, 'Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA', FEBS Letters, vol. 586, no. 4, pp. 356-361. https://doi.org/10.1016/j.febslet.2011.12.030
Seong KM, Jung SO, Kim HD, Kim HJ, Jung YJ, Choi SY et al. Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA. FEBS Letters. 2012 Feb 17;586(4):356-361. https://doi.org/10.1016/j.febslet.2011.12.030
Seong, Ki Moon ; Jung, Sang Oun ; Kim, Hag Dong ; Kim, Hee Ju ; Jung, You Jin ; Choi, Soo Young ; Kim, Joon. / Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA. In: FEBS Letters. 2012 ; Vol. 586, No. 4. pp. 356-361.
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