Abstract
Zinc, a component of a large number of enzymes and an abundant and essential trace metal, alters the structure of AGCT sites in negatively supercoiled DNA. Related sequences show little or no effect. Cobalt and cadmium show reactivities similar to zinc. The conformational change in DNA was fine mapped with haloacetaldehydes using chemical cleavage or primer extension methods. Since a many fold excess of zinc to AGCT sites is required to saturate the reaction, we propose that the zinc-AGCT complex is preferentially accessible to the haloacetaldehyde probes due to a conformational deformation. Hence, zinc is the smallest ligand (by ~500- fold) that causes a perturbation of a specific DNA sequence.
| Original language | English |
|---|---|
| Pages (from-to) | 9528-9532 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 269 |
| Issue number | 13 |
| Publication status | Published - 1994 Apr 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Zinc destabilizes DNA Watson-Crick pairs at AGCT. / Kang, Seong Man; Wells, R. D.
In: Journal of Biological Chemistry, Vol. 269, No. 13, 01.04.1994, p. 9528-9532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zinc destabilizes DNA Watson-Crick pairs at AGCT
AU - Kang, Seong Man
AU - Wells, R. D.
PY - 1994/4/1
Y1 - 1994/4/1
N2 - Zinc, a component of a large number of enzymes and an abundant and essential trace metal, alters the structure of AGCT sites in negatively supercoiled DNA. Related sequences show little or no effect. Cobalt and cadmium show reactivities similar to zinc. The conformational change in DNA was fine mapped with haloacetaldehydes using chemical cleavage or primer extension methods. Since a many fold excess of zinc to AGCT sites is required to saturate the reaction, we propose that the zinc-AGCT complex is preferentially accessible to the haloacetaldehyde probes due to a conformational deformation. Hence, zinc is the smallest ligand (by ~500- fold) that causes a perturbation of a specific DNA sequence.
AB - Zinc, a component of a large number of enzymes and an abundant and essential trace metal, alters the structure of AGCT sites in negatively supercoiled DNA. Related sequences show little or no effect. Cobalt and cadmium show reactivities similar to zinc. The conformational change in DNA was fine mapped with haloacetaldehydes using chemical cleavage or primer extension methods. Since a many fold excess of zinc to AGCT sites is required to saturate the reaction, we propose that the zinc-AGCT complex is preferentially accessible to the haloacetaldehyde probes due to a conformational deformation. Hence, zinc is the smallest ligand (by ~500- fold) that causes a perturbation of a specific DNA sequence.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028307048&partnerID=8YFLogxK
M3 - Article
C2 - 8144538
AN - SCOPUS:0028307048
VL - 269
SP - 9528
EP - 9532
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 13
ER -