ZnO nanoparticle induces apoptosis by ROS triggered mitochondrial pathway in human keratinocytes

Woo In Ryu, Yoon Hee Park, Hyun Cheol Bae, Jin Hee Kim, Sang Hoon Jeong, Hana Lee, Sang Wook Son

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Zinc Oxide nanoparticles (ZnO NPs) in its small size with large reactive surfaces can lead to toxicological injury by generating reactive oxygen species (ROS) and oxidative stress. Recently, ZnO NPs were shown to play a role in acute or chronic toxicities with mammalian cells, where its mechanism of toxicity is not fully characterized yet. In this study, the potential mechanisms of ZnO NPs in inducing and increasing oxidative stress for causing cellular apoptosis were investigated with human keratinocytes. Indeed, ZnO NPs induced significant intracellular ROS and mitochondrial ROS productions. And it seemed that cellular ROS levels induced by ZnO NPs led to the dissipation of the mitochondrial membrane potentials and elicited the cellular apoptosis. The induced ROS production by ZnO NPs was blocked with chelator treatment, which inhibited the ZnO ion. The present study demonstrates that increased levels of ROS by ZnO NPs cause mitochondrial dysfunction and cellular apoptosis.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalMolecular and Cellular Toxicology
Volume10
Issue number4
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Zinc Oxide
Keratinocytes
Nanoparticles
Reactive Oxygen Species
Apoptosis
Oxidative stress
Toxicity
Oxidative Stress
Mitochondrial Membrane Potential
Chelating Agents
Toxicology
Cells
Ions
Membranes
Wounds and Injuries

Keywords

  • Cytotoxicity
  • Keratinocyte
  • Nanoparticle
  • Reactive oxygen species
  • Zinc Oxide

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pathology and Forensic Medicine

Cite this

ZnO nanoparticle induces apoptosis by ROS triggered mitochondrial pathway in human keratinocytes. / Ryu, Woo In; Park, Yoon Hee; Bae, Hyun Cheol; Kim, Jin Hee; Jeong, Sang Hoon; Lee, Hana; Son, Sang Wook.

In: Molecular and Cellular Toxicology, Vol. 10, No. 4, 01.01.2015, p. 387-391.

Research output: Contribution to journalArticle

Ryu, Woo In ; Park, Yoon Hee ; Bae, Hyun Cheol ; Kim, Jin Hee ; Jeong, Sang Hoon ; Lee, Hana ; Son, Sang Wook. / ZnO nanoparticle induces apoptosis by ROS triggered mitochondrial pathway in human keratinocytes. In: Molecular and Cellular Toxicology. 2015 ; Vol. 10, No. 4. pp. 387-391.
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AU - Son, Sang Wook

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