ZnO nanorod matrix based electrochemical immunosensors for sensitivity enhanced detection of Legionella pneumophila

Jungil Park, Xueqiu You, Yunseok Jang, Yoonkyung Nam, Min Ja Kim, Nam Ki Min, James Jungho Pak

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An ultrasensitive electrochemical immunosensor based on a ZnO nanorod (NR) matrix electrode was developed for detecting Legionella pneumophila for the first time. The ZnO NR matrix electrode was grown on an underlying Au working electrode (dia. 1.6 mm) using a hydrothermal method at a low temperature. A sandwich enzyme-linked immunosorbent assay was performed using the fabricated immunosensor. A primary antibody (1-4 μg/mL) was immobilized onto the ZnO NR matrix electrode by electrostatic interaction and the peptidoglycan-associated lipoprotein of L. pneumophila, as a component of Legionella antigen (1-5000 pg/mL) was bound to the primary antibody. A secondary antibody (1-4 μg/mL) conjugated to horseradish peroxidase was then bound to the antigen. The fabricated immunosensor was evaluated in a 0.1 M acetate buffer (pH 5) containing 0.05 mM 3,3′,5,5′-tetramethylbenzidine and 1 mM H 2O2 by cyclic voltammetry. In the fabricated immunosensors based on 1 μg/mL and 2 μg/mL antibody concentrations, the current output logarithmically increased with the L. pneumophila antigen concentrations from 1 to 5000 pg/mL. The detection limit is ∼1 pg/mL (S/N = 4) with excellent selectivity.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume200
DOIs
Publication statusPublished - 2014 Sep

Keywords

  • Electrochemistry
  • Immunosensor
  • Legionella pneumophila
  • Nanorods
  • Zinc oxide

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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