Abstract
A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.
| Original language | English |
|---|---|
| Article number | 002825 |
| Pages (from-to) | 2279-2284 |
| Number of pages | 6 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 68 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2018 Jul 1 |
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Keywords
- Beach sediment
- Gammaproteobacteria
- PAH degradation
- Zobellella
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment. / Lee, Dong Wan; Lee, Hanbyul; Kwon, Bong Oh; Khim, Jong Seong; Yim, Un Hyuk; Park, Hongjae; Park, Byeonghyeok; Choi, In-Geol; Kim, Beom Seok; Kim, Jae-Jin.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 7, 002825, 01.07.2018, p. 2279-2284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment
AU - Lee, Dong Wan
AU - Lee, Hanbyul
AU - Kwon, Bong Oh
AU - Khim, Jong Seong
AU - Yim, Un Hyuk
AU - Park, Hongjae
AU - Park, Byeonghyeok
AU - Choi, In-Geol
AU - Kim, Beom Seok
AU - Kim, Jae-Jin
PY - 2018/7/1
Y1 - 2018/7/1
N2 - A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.
AB - A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.
KW - Beach sediment
KW - Gammaproteobacteria
KW - PAH degradation
KW - Zobellella
UR - http://www.scopus.com/inward/record.url?scp=85049918692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049918692&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002825
DO - 10.1099/ijsem.0.002825
M3 - Article
C2 - 29781802
AN - SCOPUS:85049918692
VL - 68
SP - 2279
EP - 2284
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 7
M1 - 002825
ER -