Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

Dong Wan Lee, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Hongjae Park, Byeonghyeok Park, In-Geol Choi, Beom Seok Kim, Jae-Jin Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.

Original languageEnglish
Article number002825
Pages (from-to)2279-2284
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number7
DOIs
Publication statusPublished - 2018 Jul 1

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Polycyclic Aromatic Hydrocarbons
polycyclic aromatic hydrocarbons
rRNA Genes
beaches
PAH
beach
Bacteria
Lipids
sediments
Republic of Korea
Phosphatidylglycerols
bacterium
bacteria
Base Composition
lipid
Catalase
sediment
Names
Sequence Analysis
Oxidoreductases

Keywords

  • Beach sediment
  • Gammaproteobacteria
  • PAH degradation
  • Zobellella

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment. / Lee, Dong Wan; Lee, Hanbyul; Kwon, Bong Oh; Khim, Jong Seong; Yim, Un Hyuk; Park, Hongjae; Park, Byeonghyeok; Choi, In-Geol; Kim, Beom Seok; Kim, Jae-Jin.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 7, 002825, 01.07.2018, p. 2279-2284.

Research output: Contribution to journalArticle

Lee, Dong Wan ; Lee, Hanbyul ; Kwon, Bong Oh ; Khim, Jong Seong ; Yim, Un Hyuk ; Park, Hongjae ; Park, Byeonghyeok ; Choi, In-Geol ; Kim, Beom Seok ; Kim, Jae-Jin. / Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 7. pp. 2279-2284.
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abstract = "A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 {\%} (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 {\%} 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 {\%}) and Zobellella taiwanensis ZT1T (96.0 {\%}). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol{\%}. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 {\%}. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.",
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AU - Khim, Jong Seong

AU - Yim, Un Hyuk

AU - Park, Hongjae

AU - Park, Byeonghyeok

AU - Choi, In-Geol

AU - Kim, Beom Seok

AU - Kim, Jae-Jin

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AB - A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.

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