Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

Dong Wan Lee; Hanbyul Lee; Bong Oh Kwon; Jong Seong Khim; Un Hyuk Yim; Hongjae Park; Byeonghyeok Park; In Geol Choi; Beom Seok Kim; Jae Jin Kim

doi:10.1099/ijsem.0.002825

Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

Dong Wan Lee, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Hongjae Park, Byeonghyeok Park, In Geol Choi, Beom Seok Kim, Jae Jin Kim

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4^T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4^T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671^T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1^T (96.4 %) and Zobellella taiwanensis ZT1^T (96.0 %). The major fatty acids were summed feature 3 (comprising C_{16: 1}ω7c and/or C_{16: 1}ω6c), C_{12: 0}, summed feature 8 (comprising C_{18: 1}ω7c and/or C_{18: 1}ω6c) and C_{16: 0}The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4^T and Z. aerophila JC2671^T was 12.4±1.3 %. The phenotypic properties of 102-Py4^T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4^T (=KCTC 62272^T=JCM 32359^T=DSM 106043^T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.

Original language	English
Article number	002825
Pages (from-to)	2279-2284
Number of pages	6
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	68
Issue number	7
DOIs	https://doi.org/10.1099/ijsem.0.002825
Publication status	Published - 2018 Jul

Keywords

Beach sediment
Gammaproteobacteria
PAH degradation
Zobellella

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijsem.0.002825

Cite this

Lee, D. W., Lee, H., Kwon, B. O., Khim, J. S., Yim, U. H., Park, H., Park, B., Choi, I. G., Kim, B. S., & Kim, J. J. (2018). Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment. International Journal of Systematic and Evolutionary Microbiology, 68(7), 2279-2284. [002825]. https://doi.org/10.1099/ijsem.0.002825

@article{bf7108676aca45baa056bdad5347b7bc,

title = "Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment",

abstract = "A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.",

keywords = "Beach sediment, Gammaproteobacteria, PAH degradation, Zobellella",

author = "Lee, {Dong Wan} and Hanbyul Lee and Kwon, {Bong Oh} and Khim, {Jong Seong} and Yim, {Un Hyuk} and Hongjae Park and Byeonghyeok Park and Choi, {In Geol} and Kim, {Beom Seok} and Kim, {Jae Jin}",

note = "Funding Information: This work was supported by the project entitled {\textquoteleft}Oil Spill Environmental Impact Assessment and Environmental Restoration (PM57431){\textquoteright} funded by the Ministry of Oceans and Fisheries of Korea. Publisher Copyright: {\textcopyright} 2018 IUMS.",

year = "2018",

month = jul,

doi = "10.1099/ijsem.0.002825",

language = "English",

volume = "68",

pages = "2279--2284",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

number = "7",

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T1 - Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

AU - Lee, Dong Wan

AU - Lee, Hanbyul

AU - Kwon, Bong Oh

AU - Khim, Jong Seong

AU - Yim, Un Hyuk

AU - Park, Hongjae

AU - Park, Byeonghyeok

AU - Choi, In Geol

AU - Kim, Beom Seok

AU - Kim, Jae Jin

N1 - Funding Information: This work was supported by the project entitled ‘Oil Spill Environmental Impact Assessment and Environmental Restoration (PM57431)’ funded by the Ministry of Oceans and Fisheries of Korea. Publisher Copyright: © 2018 IUMS.

PY - 2018/7

Y1 - 2018/7

N2 - A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.

AB - A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4T, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1–3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4T clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671T (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1T (96.4 %) and Zobellella taiwanensis ZT1T (96.0 %). The major fatty acids were summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C12: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C16: 0The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. The DNA–DNA relatedness value between strain 102-Py4T and Z. aerophila JC2671T was 12.4±1.3 %. The phenotypic properties of 102-Py4T demonstrated that this strain could be distinguished from other Zobellella species. On the basis of the data presented, strain 102-Py4T (=KCTC 62272T=JCM 32359T=DSM 106043T) is considered to represent a novel species of the genus Zobellella, for which the name Zobellella maritima sp. nov. is proposed.

KW - Beach sediment

KW - Gammaproteobacteria

KW - PAH degradation

KW - Zobellella

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U2 - 10.1099/ijsem.0.002825

DO - 10.1099/ijsem.0.002825

M3 - Article

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AN - SCOPUS:85049918692

VL - 68

SP - 2279

EP - 2284

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 7

M1 - 002825

ER -

Zobellella maritima sp. Nov., a polycyclic aromatic hydrocarbon-degrading bacterium, isolated from beach sediment

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