Zoogloea caeni sp. nov., a floc-forming bacterium isolated from activated sludge

Yongqi Shao, Bok Sil Chung, Seung Sik Lee, Woojun Park, Sang Suk Lee, Che Ok Jeon

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Two floc-forming, nitrogen-fixing bacteria, strains EMB43T and EMB61, obtained from activated sludge of a domestic wastewater treatment plant in Korea, were characterized. The two strains were very closely related, sharing 99.7 % 16S rRNA gene sequence similarity and showing a level of DNA-DNA relatedness of 93 %, which suggests that they represent members of a single species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two novel isolates formed a distinct phyletic lineage within the genus Zoogloea and were related most closely to Zoogloea resiniphila DhA-35T and Zoogloea oryzae A-7T, with sequence similarities of 97.2 %. Levels of DNA-DNA relatedness between strain EMB43T and Z resiniphila DhA-35T and Z oryzae A-7T were 12.8 and 7.4 %, respectively. Cells of strains EMB43T and EMB61 were facultatively aerobic, rod-shaped, Gram-negative and motile by means of a polar flagellum. The strains grew at temperatures of 15-40 °C (optimum: 25-30 °C) and at pH 6.0-9.0 (optimum: pH 6.5-7.5). The predominant fatty acids were C16:0, C10:0 3-OH and summed feature 3 (C16:1ω7c and/or iso_C15:0 2-OH), and the predominant polar lipid was phosphatidylethanolamine. The genomic DNA G + C content was 64.9-65.0 mol% and the major isoprenoid quinone was ubiquinone-8 (Q-8). On the basis of phenotypic, chemotaxonomic and molecular data, the isolates are considered to represent a novel species of the genus Zoogloea, for which the name Zoogloea caeni sp. nov. is proposed. The type strain is EMB43T (=KCTC 22084T=DSM 19389T).

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number3
DOIs
Publication statusPublished - 2009 Nov 9

Fingerprint

Zoogloea
activated sludge
Sewage
Bacteria
DNA
bacterium
new species
bacteria
rRNA Genes
relatedness
Oryza
Zoogloea resiniphila
Genes
ribosomal RNA
Flagella
gene
isoprenoid
Terpenes
Base Composition
nucleotide sequences

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Zoogloea caeni sp. nov., a floc-forming bacterium isolated from activated sludge. / Shao, Yongqi; Chung, Bok Sil; Lee, Seung Sik; Park, Woojun; Lee, Sang Suk; Jeon, Che Ok.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 59, No. 3, 09.11.2009, p. 526-530.

Research output: Contribution to journalArticle

Shao, Yongqi ; Chung, Bok Sil ; Lee, Seung Sik ; Park, Woojun ; Lee, Sang Suk ; Jeon, Che Ok. / Zoogloea caeni sp. nov., a floc-forming bacterium isolated from activated sludge. In: International Journal of Systematic and Evolutionary Microbiology. 2009 ; Vol. 59, No. 3. pp. 526-530.
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