Abstract
Two red pigment-producing bacterial strains with a metallic green sheen were isolated from a sediment sample of getbol, the Korean tidal flat. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates represent a phyletic lineage within the γ-Proteobacteria that is distantly related to the genus Hahella. No bacterial species with validly published names showed ≥92% 16S rRNA similarity with the getbol isolates. The strains were Gram-negative, chemo-organotrophic, aerobic and required NaCl (1-7%) for growth. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The major isoprenoid quinone was ubiquinone-9. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. The DNA G+C contents ranged from 40 to 42 mol%. The combination of physiological, biochemical and chemotaxonomic data clearly separated the test strains from other phylogenetically related genera in the γ-Proteobacteria. On the basis of polyphasic evidence from this study, it is proposed that the two getbol isolates should be classified in a novel genus, Zooshikella gen. nov., as Zooshikella ganghwensis sp. nov.
| Original language | English |
|---|---|
| Pages (from-to) | 1013-1018 |
| Number of pages | 6 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 53 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2003 Jul 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Applied Microbiology and Biotechnology
- Immunology
- Microbiology
Cite this
Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. / Yi, Hana; Chang, Young H.; Oh, Hyun Woo; Bae, Kyung Sook; Chun, Jongsik.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 53, No. 4, 01.07.2003, p. 1013-1018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments
AU - Yi, Hana
AU - Chang, Young H.
AU - Oh, Hyun Woo
AU - Bae, Kyung Sook
AU - Chun, Jongsik
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Two red pigment-producing bacterial strains with a metallic green sheen were isolated from a sediment sample of getbol, the Korean tidal flat. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates represent a phyletic lineage within the γ-Proteobacteria that is distantly related to the genus Hahella. No bacterial species with validly published names showed ≥92% 16S rRNA similarity with the getbol isolates. The strains were Gram-negative, chemo-organotrophic, aerobic and required NaCl (1-7%) for growth. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The major isoprenoid quinone was ubiquinone-9. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. The DNA G+C contents ranged from 40 to 42 mol%. The combination of physiological, biochemical and chemotaxonomic data clearly separated the test strains from other phylogenetically related genera in the γ-Proteobacteria. On the basis of polyphasic evidence from this study, it is proposed that the two getbol isolates should be classified in a novel genus, Zooshikella gen. nov., as Zooshikella ganghwensis sp. nov.
AB - Two red pigment-producing bacterial strains with a metallic green sheen were isolated from a sediment sample of getbol, the Korean tidal flat. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates represent a phyletic lineage within the γ-Proteobacteria that is distantly related to the genus Hahella. No bacterial species with validly published names showed ≥92% 16S rRNA similarity with the getbol isolates. The strains were Gram-negative, chemo-organotrophic, aerobic and required NaCl (1-7%) for growth. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The major isoprenoid quinone was ubiquinone-9. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. The DNA G+C contents ranged from 40 to 42 mol%. The combination of physiological, biochemical and chemotaxonomic data clearly separated the test strains from other phylogenetically related genera in the γ-Proteobacteria. On the basis of polyphasic evidence from this study, it is proposed that the two getbol isolates should be classified in a novel genus, Zooshikella gen. nov., as Zooshikella ganghwensis sp. nov.
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U2 - 10.1099/ijs.0.02521-0
DO - 10.1099/ijs.0.02521-0
M3 - Article
VL - 53
SP - 1013
EP - 1018
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 4
ER -